ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans
- Authors
- Kieffer, Nicolas; Guzmán-Puche, Julia; Poirel, Laurent; Kang, Hyo Jung; Jeon, Che Ok; Nordmann, Patrice
- Issue Date
- Jun-2019
- Publisher
- NLM (Medline)
- Citation
- The Journal of antimicrobial chemotherapy, v.74, no.6, pp 1568 - 1571
- Pages
- 4
- Journal Title
- The Journal of antimicrobial chemotherapy
- Volume
- 74
- Number
- 6
- Start Page
- 1568
- End Page
- 1571
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33156
- DOI
- 10.1093/jac/dkz057
- ISSN
- 1460-2091
1460-2091
- Abstract
- OBJECTIVES: Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment. METHODS: The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme. RESULTS: The enzyme named ZHO-1 shared 51% amino acid identity with the acquired class B carbapenemases IMP-1, KHM-1 and DIM-1. Expression of the blaZHO-1 gene in a susceptible E. coli resulted in a carbapenemase phenotype. Kinetic parameters determined from purified ZHO-1 enzyme showed that it had significant hydrolytic activity against most β-lactams including penicillins, cephalosporins and carbapenems, with the exception of aztreonam and cefepime. CONCLUSIONS: This study adds to the knowledge regarding environmental species as a reservoir of possible clinically relevant MBLs. © The Author(s) 2019. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com.
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