카드뮴 유도 잉어 혈액으로부터의 Metallothionein 정제 및 이에 대한 다클론 항체 생산Purification of Carp Serum Metallothionein Induced in Carp with Cadmium and Production of Polyclonal Antibody
- Authors
- 유형석; 강호준; 김슬기; 김남수; 김우연
- Issue Date
- 2009
- Publisher
- 한국산업식품공학회
- Keywords
- carp metallothionein; purification; antibody; reactivity
- Citation
- 산업식품공학, v.13, no.4, pp 314 - 319
- Pages
- 6
- Journal Title
- 산업식품공학
- Volume
- 13
- Number
- 4
- Start Page
- 314
- End Page
- 319
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33306
- ISSN
- 1226-4768
- Abstract
- Metallothioneins (MTs) are low molecular weight, cysteine-rich, and heavy metal binding proteins, which could be
induced with heavy metals such as Cd, Hg, Zn and Cu in liver, kidney, and in cultured cells. By using ion exchange
chromatography on DE-52, MT was purified from the serum of carp induced with cadmium in order to produce
antibody against MT. Polyclonal antibody produced against purified carp MT reacted well with MT in the serum
of carp induced with cadmium, whereas control serum did not. This may indicate that the polyclonal antibody
against the carp MT could be used for the preparation of biosensors to detect MT in fishes like carp.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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