고속액체크로마토그래피를 이용한 가시오갈피의 리그난 함량Lignan contents in Acanthopanax senticosus by HPLC
- Authors
- 김혜민; 조선행; 국순자; 이상현
- Issue Date
- Sep-2011
- Publisher
- 충남대학교 농업과학연구소
- Keywords
- Acanthopanax senticosus; Eleutherosides B and E; HPLC; Water extract
- Citation
- Korean Journal of Agricultural Science, v.38, no.3, pp 479 - 484
- Pages
- 6
- Journal Title
- Korean Journal of Agricultural Science
- Volume
- 38
- Number
- 3
- Start Page
- 479
- End Page
- 484
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33843
- ISSN
- 2466-2402
2466-2410
- Abstract
- A reverse-phase system of HPLC using a linear gradient of acetonitrile and deionized water was developed for the quantification lignans, eleutherosides B and E, in Acanthopanax senticosus. The HPLC system consisted of linear gradient of acetonitrile and deionized water, and UV/VIS detection was set at 210 nm. Both eleutherosides B and E contents in different parts of A. senticosus were determined. As a result, the contents of eleutherosides B and E were measured in the leaves (trace amounts and 0.029 mg/g, respectively), stems (0.107 and 1.015 mg/g, respectively), roots (0.026 and 0.390 mg/g, respectively), and fruits (0.022 and 0.043 mg/g, respectively). Moreover, eleutherosides B and E in the water extract were found 0.011 and 0.171 mg/g, respectively.
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