Steroidal Saponin을 이용한 위유, 황정의 분류 및 함량 분석법 개발Content Analysis and Classification for Polygonati Odorati Rhizoma and Polygonati Rhizoma by Steroidal Saponin
- Authors
- 김성건; 신소영; 문예지; 서지윤; 김호경; 황완균
- Issue Date
- 2010
- Publisher
- 대한약학회
- Keywords
- Polygonati rhizoma; Polygonati odorati rhizoma; steroidal saponin; origin; content analysis
- Citation
- 약 학 회 지, v.54, no.6, pp 441 - 448
- Pages
- 8
- Journal Title
- 약 학 회 지
- Volume
- 54
- Number
- 6
- Start Page
- 441
- End Page
- 448
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/34541
- ISSN
- 0377-9556
- Abstract
- In present study, classification and quality control of Genus Polygonatum were developed using the isolated from Polygonati Odorati Rhizoma and Polygonati Rhizoma. 3 components were isolated from Butanol fractions of Polygonati Rhizoma, and 2 components were isolated from Hexane and Butanol fractions of Polygonati Odorati Rhizoma. All the components were obtained using silica gel and ODS column chromatography. The compounds were identified as adenosine, 14-hydroxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranosyl-26-O-β-D-glucopyranoside,22-O-methyl-14-hydrocxyfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-O-β-Dgalactopyranosyl-26-O-β-D-glucopyranoside, β-Sitosteryl-3-O-β-D-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-β-Dglucopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(1→3)]-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranoside through physicochemical data, spectroscopic methods (1H-NMR, 13C-NMR, Mass) according references. The quality control of genus Polygonatum were conducted using HPLC quantitative analysis of 14-hydroxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranosyl-26-O-β-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(1→3)]-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranoside in 30 samples collected throughout Korea and China. This method provided a tool for standardization of mix or misusing the commercial Odorati Rhizoma and Polygonati Rhizoma. As a result, contained quantity of 14-hydroxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranosyl-26-O-β-D-glucopyranoside was measured 0.008±0.006% and 14-hydoxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(13)]-O-β-D-glucopyranosyl-(1→4)-O-β-Dgalactopyranoside was measured 0.026±0.012%.
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