Ectopic expression of the catalytic subunit of telomerase protects against brain injury resulting from ischemia and NMDA-induced neurotoxicity
- Authors
- Kang, Hyo Jung; Choi, Yoon Sik; Hong, Seung-Beom; Kim, Kee-Won; Woo, Ran-Sook; Won, Seok Joon; Kim, Eun Ju; Jeon, Hee Kyung; Jo, So-Young; Kim, Tae Kook; Bachoo, Robert; Reynolds, Ian J.; Gwag, Byoung Joo; Lee, Han-Woong
- Issue Date
- Feb-2004
- Publisher
- SOC NEUROSCIENCE
- Keywords
- catalytic subunit of telomerase; ischemia; excitotoxicity; NMDA; calcium; mitochondria
- Citation
- JOURNAL OF NEUROSCIENCE, v.24, no.6, pp 1280 - 1287
- Pages
- 8
- Journal Title
- JOURNAL OF NEUROSCIENCE
- Volume
- 24
- Number
- 6
- Start Page
- 1280
- End Page
- 1287
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/36710
- DOI
- 10.1523/JNEUROSCI.4082-03.2004
- ISSN
- 0270-6474
1529-2401
- Abstract
- The catalytic subunit of telomerase reverse transcriptase ( TERT) protects dividing cells from replicative senescence in vitro. Here, we show that expression of TERT mRNA is induced in the ipsilateral cortical neurons after occlusion of the middle cerebral artery in adult mice. Transgenic mice that overexpress TERT showed significant resistance to ischemic brain injury. Among excitotoxicity, oxidative stress, and apoptosis comprising of routes of ischemic neuronal death, NMDAreceptor-mediated excitotoxicity was reduced in forebrain cell cultures overexpressing TERT. NMDA-induced accumulation of cytosolic free Ca2+ ([Ca2+](c)) was reduced in forebrain neurons from TERT transgenic mice, which was attributable to the rapid flow of [Ca2+](c) into the mitochondria from the cytosol without change in Ca2+ influx and efflux through the plasma membrane. The present study provides evidence that TERT is inducible in postmitotic neurons after ischemic brain injury and prevents NMDA neurotoxicity through shift of the cytosolic free Ca2+ into the mitochondria, and thus plays a protective role in ameliorating ischemic neuronal cell death.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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