벼 렉틴 유전자의 클로닝 및 대장균에서의 발현
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 홍성관 | - |
dc.contributor.author | 전상훈 | - |
dc.contributor.author | 김하형 | - |
dc.contributor.author | 공광훈 | - |
dc.date.available | 2019-10-14T01:40:08Z | - |
dc.date.issued | 2002-08 | - |
dc.identifier.issn | 0377-9556 | - |
dc.identifier.issn | 2383-9457 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/36798 | - |
dc.description.abstract | The lectin gene from rice was amplified by the polymerase chain reaction. The amplified DNA was inserted into the expression vector pET26b and expressed it as a fusion protein with polyhistidine sequences in Escherichia coli. The recombinant protein was produced by induction with 0.4 mM isopropyl- β -D-thiogalactopyranoside at 37 ℃ and purified by an immobilized metal affinity chromatography. The recombinant protein was found to have lectin activity by the hemagglutination inhibition assay. The hemagglutination activity of the recombinant protein was optimal at pH 4.0-7.0 and was dependent on Ca2+ and Mn 2+. | - |
dc.format.extent | 6 | - |
dc.publisher | 대한약학회 | - |
dc.title | 벼 렉틴 유전자의 클로닝 및 대장균에서의 발현 | - |
dc.title.alternative | Molecular Cloning and Expression of Rice Lectin in Escherichia coli | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | 약 학 회 지, v.46, no.4, pp 270 - 275 | - |
dc.identifier.kciid | ART000881357 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 275 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 270 | - |
dc.citation.title | 약 학 회 지 | - |
dc.citation.volume | 46 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Lectin | - |
dc.subject.keywordAuthor | rice | - |
dc.subject.keywordAuthor | gene cloning | - |
dc.subject.keywordAuthor | expression in E.coli | - |
dc.description.journalRegisteredClass | kciCandi | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.