Requirement of Pretone by Thromboxane A2 for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat
- Authors
- Park, Su Jung; Yoo, Hae Young; Kim, Hye Jin; Kim, Jin Kyoung; Zhang, Yin-Hua; Kim, Sung Joon
- Issue Date
- Feb-2012
- Publisher
- 대한약리학회
- Keywords
- Lung slice; Hypoxic pulmonary vasoconstriction; Thromboxane A2; Airway smooth muscle
- Citation
- The Korean Journal of Physiology & Pharmacology, v.16, no.1, pp 59 - 64
- Pages
- 6
- Journal Title
- The Korean Journal of Physiology & Pharmacology
- Volume
- 16
- Number
- 1
- Start Page
- 59
- End Page
- 64
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/37230
- ISSN
- 1226-4512
2093-3827
- Abstract
- Hypoxic pulmonary vasoconstriction (HPV) is physiologically important response for preventing mismatching between ventilation and perfusion in lungs. The HPV of isolated pulmonary arteries (HPV-PA) usually require a partial pretone by thromboxane agonist (U46619). Because the HPV of ventilated/perfused lungs (HPV-lung) can be triggered without pretone conditioning, we suspected that a putative tissue factor might be responsible for the pretone of HPV. Here we investigated whether HPV can be also observed in precision-cut lung slices (PCLS) from rats. The HPV in PCLS also required partial contraction by U46619. In addition, K+ channel blockers (4AP and TEA) required U46619-pretone to induce significant contraction of PA in PCLS. In contrast, the airways in PCLS showed reversible contraction in response to the K+ channel blockers without pretone conditioning. Also, the airways showed no hypoxic constriction but a relaxation under the partial pretone by U46619. The airways in PCLS showed reliable, concentration-dependent contraction by metacholine (EC50, ∼210nM). In summary, the HPV in PCLS is more similar to isolated PA than V/P lungs. The metacholineinduced constriction of bronchioles suggested that the PLCS might be also useful for studying airway physiology in situ.
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Collections - Red Cross College of Nursing > Department of Nursing > 1. Journal Articles
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