Dynamic changes of oligomeric amyloid beta levels in plasma induced by spiked synthetic A beta(42)open access
- Authors
- An, Seong Soo A.; Lee, Byoung-sub; Yu, Ji Sun; Lim, Kuntaek; Kim, Gwang Je; Lee, Ryan; Kim, Shinwon; Kang, Sungmin; Park, Young Ho; Wang, Min Jeong; Yang, Young Soon; Youn, Young Chul; Kim, SangYun
- Issue Date
- Oct-2017
- Publisher
- BIOMED CENTRAL LTD
- Keywords
- Multimer detection system; Alzheimer's disease; Amyloid-beta; Oligomers; Blood biomarker; Synthetic amyloid-beta; ELISA; Plasma test
- Citation
- ALZHEIMERS RESEARCH & THERAPY, v.9, no.1
- Journal Title
- ALZHEIMERS RESEARCH & THERAPY
- Volume
- 9
- Number
- 1
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3771
- DOI
- 10.1186/s13195-017-0310-6
- ISSN
- 1758-9193
- Abstract
- Background: A reliable blood-based assay is required to properly diagnose and monitor Alzheimer's disease (AD). Many attempts have been made to develop such a diagnostic tool by measuring amyloid-beta oligomers (A beta Os) in the blood, but none have been successful in terms of method reliability. We present a multimer detection system (MDS), initially developed for the detection of prion oligomers in the blood, to detect A beta Os. Methods: To characterize A beta in the blood, plasma was spiked with synthetic amyloid-beta (A beta) and incubated over time. Then, the MDS was used to monitor the dynamic changes of A beta O levels in the plasma. Results: Increasing concentrations of A beta Os were observed in the plasma of patients with AD but not in the plasma of normal control subjects. The plasma from patients with AD (n = 27) was differentiated from that of the age-matched normal control subjects (n = 144) with a sensitivity of 83.3% and a specificity of 90.0%. Conclusions: Synthetic A beta spiked into the blood plasma of patients with AD, but that of not elderly normal control subjects, induced dynamic changes in the formation of A beta Os over time. A beta Os were detected by the MDS, which is a useful blood-based assay with high sensitivity and specificity for AD diagnosis.
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