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Silk fibroin scaffolds potentiate immunomodulatory function of human mesenchymal stromal cells

Authors
Kim, Ok-HyeonYoon, Ok JaLee, Hyun Jung
Issue Date
Nov-2019
Publisher
Elsevier B.V.
Keywords
IDO; Immunomodulation; Mesenchymal stromal cell; Regenerative medicine; Silk fibroin nanofiber
Citation
Biochemical and Biophysical Research Communications, v.519, no.2, pp 323 - 329
Pages
7
Journal Title
Biochemical and Biophysical Research Communications
Volume
519
Number
2
Start Page
323
End Page
329
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/37710
DOI
10.1016/j.bbrc.2019.09.006
ISSN
0006-291X
1090-2104
Abstract
Although mesenchymal stromal cells (MSCs) show great potential for use in regenerative medicine, their therapeutic efficacy remains limited because of their low adaptation efficiency and viability observed in clinical trials. To potentiate the adaptation and survival efficiency of MSCs after administration in vivo, silk fibroin nanofibers (SFNs) were applied as a scaffold. SFNs are biocompatible, biodegradable polymers with tunable architectures and mechanical properties. Treatment with interferon (IFN)−γ for 18 h increased the expression of immunomodulatory functional cytokines, IDO and COX2 in MSCs. Further, the MSCs grown on SFN sheets showed enhanced IDO1 and COX2 expression following IFN-γ treatment. MSCs showed significantly greater migratory ability on SFN sheets than on glass surfaces or PLGA control sheets. Though IFN-γ treatment slightly reduced the migration ability of MSCs cultured on glass or poly(lactic-co-glycolic acid) (PLGA) nanofiber sheets, it did not alter MSC motility on SFN sheets. Furthermore, MSCs cultured on SFN sheets dramatically suppressed TNF-α secretion from lipopolysaccharide-activated murine splenocytes, suggesting that the immunomodulatory function of MSCs was enhanced by the SFN sheets. Taken together, these data demonstrate that SFN sheets potentiate the reparative and regenerative properties of MSCs. © 2019 The Authors
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