Recent advances in genetic engineering tools based on synthetic biology
- Authors
- Ren, Jun; Lee, Jingyu; Na, Dokyun
- Issue Date
- Jan-2020
- Publisher
- Microbiological Society of Korea
- Keywords
- CRISPR; CRISPRa; CRISPRi; genome-scale engineering; MAGE; metabolic engineering; promoter engineering; synthetic sRNA
- Citation
- Journal of Microbiology, v.58, no.1, pp 1 - 10
- Pages
- 10
- Journal Title
- Journal of Microbiology
- Volume
- 58
- Number
- 1
- Start Page
- 1
- End Page
- 10
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38231
- DOI
- 10.1007/s12275-020-9334-x
- ISSN
- 1225-8873
1976-3794
- Abstract
- Genome-scale engineering is a crucial methodology to rationally regulate microbiological system operations, leading to expected biological behaviors or enhanced bioproduct yields. Over the past decade, innovative genome modification technologies have been developed for effectively regulating and manipulating genes at the genome level. Here, we discuss the current genome-scale engineering technologies used for microbial engineering. Recently developed strategies, such as clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9, multiplex automated genome engineering (MAGE), promoter engineering, CRISPR-based regulations, and synthetic small regulatory RNA (sRNA)-based knockdown, are considered as powerful tools for genome-scale engineering in microbiological systems. MAGE, which modifies specific nucleotides of the genome sequence, is utilized as a genome-editing tool. Contrastingly, synthetic sRNA, CRISPRi, and CRISPRa are mainly used to regulate gene expression without modifying the genome sequence. This review introduces the recent genome-scale editing and regulating technologies and their applications in metabolic engineering. © 2020, The Microbiological Society of Korea.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of ICT Engineering > School of Integrative Engineering > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38231)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.