쓴메밀 분획물과 Rutin의 라디칼 소거능 및 C6 Glioma 세포의 산화적 스트레스 개선 효과

Abstract

The protective effects from oxidative stress of fractions and rutin from Fagopyrum tataricum were evaluated. The treatment of n-hexane, methylene chloride, ethyl acetate, and n-butanol fractions from F. tataricum showed over 90% scavenging effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) at 500μg/ml. In addition, the fractions showed over 80% hydroxyl radical (ㆍOH) scavenging activity at the concentration of 50μg/ml. Moreover, active compound, rutin, showed over 80% scavenging effect against DPPH at 50μg/ml. ㆍOH scavenging activity of rutin showed over 80% at 10μg/ml. Furthermore, the protective effect against oxidative stress using C6 glioma cell was also studied. The treatment of H2O2to C6 glioma cell induced oxidative stress, however the fractions from F. tataricum and rutin increased significantly the cell viability. The present results indicate that the fractions from F. tataricum and active compound, rutin, showed protective effects against oxidative stress.