Chryseobacterium mulctrae sp. nov., isolated from raw cow's milk

Abstract

A Gram-stain-negative bacterial strain, designated CA10(T), was isolated from bovine raw milk sampled in Anseong, Republic of Korea. Cells were yellow-pigmented, aerobic, non-motile bacilli and grew optimally at 30 degrees C and pH 7.0 on tryptic soy agar without supplementation of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain CA10(T) belonged to the genus Chryseobacterium , family Flavobacteriaceae , and was most closely related to Chryseobacterium indoltheticum ATCC 27950(T) (98.75 % similarity). The average nucleotide identity and digital DNA-DNA hybridization values of strain CA10(T) were 94.4 and 56.9 %, respectively, relative to Chryseobacterium scophthalmum DSM 16779(T), being lower than the cut-off values of 95-96 and 70 %, respectively. The predominant respiratory quinone was menaquinone-6; major polar lipid, phosphatidylethanolamine; major fatty acids, iso-C-15 : 0 , summed feature 9 (iso-C-17 : 1 omega 9c and/or C(16 : 0 )10-methyl), summed feature 3 (iso-C-15 : 0 2-OH and/or C-16 : 1 omega 7c) and iso-C-17 : 0 3-OH. The results of physiological, chemotaxonomic and biochemical analyses suggested that strain CA10(T) is a novel species of genus Chryseobacterium , for which the name Chryseobacterium mulctrae sp. nov. is proposed. The type strain is CA10(T) (=KACC 21234(T)=JCM 33443(T)).