β-Hydroxybutyrate dehydrogenase decorated MXene nanosheets for the amperometric determination of β-hydroxybutyrate
- Authors
- Koyappayil, Aneesh; Chavan, Sachin Ganpat; Mohammadniaei, Mohsen; Go, Anna; Hwang, Sei Young; Lee, Min-Ho
- Issue Date
- 20-Apr-2020
- Publisher
- SPRINGER WIEN
- Keywords
- MXene; Ti3C2Tx; Ti3AlC2; beta-Hydroxybutyrate; Biosensor; Amperometric sensor; Ketone bodies; Diabetic ketosis; Diabetic ketoacidosis
- Citation
- MICROCHIMICA ACTA, v.187, no.5
- Journal Title
- MICROCHIMICA ACTA
- Volume
- 187
- Number
- 5
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/41922
- DOI
- 10.1007/s00604-020-04258-y
- ISSN
- 0026-3672
1436-5073
- Abstract
- MXene nanosheets of type Ti3C2Tx were modified with beta-hydroxybutyrate dehydrogenase and then used as a biosensor for amperometric sensing of beta-hydroxybutyrate. The MXene and the nanocomposite were characterized by X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The MXene has a layered structure and proved to be an excellent immobilization matrix providing good compatibility with the enzyme beta-hydroxybutyrate dehydrogenase. The MXene-based biosensor, best operated at a potential of - 0.35 V (vs. Ag/AgCl), displays a wide linear range (0.36 to 17.9 mM), a sensitivity of 0.480 mu A mM(-1) cm(-2), and a low detection limit (45 mu M). The biosensor was successfully applied to the determination of beta-hydroxybutyrate in (spiked) real serum samples.
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