n-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR-pMHC Interaction of Anti-Viral CD8+ T Cellsopen access
- Authors
- Lim, Younghyun; Kim, Seyoung; Kim, Sehoon; Kim, Dong-In; Kang, Kyung Won; Hong, So-Hee; Lee, Sang-Myeong; Koh, Hye Ran; Seo, Young-Jin
- Issue Date
- Jun-2020
- Publisher
- NLM (Medline)
- Keywords
- omega-3; CD8+ T cells; LCMV; TCR-pMHC interaction
- Citation
- Viruses, v.12, no.6
- Journal Title
- Viruses
- Volume
- 12
- Number
- 6
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/42719
- DOI
- 10.3390/v12060639
- ISSN
- 1999-4915
1999-4915
- Abstract
- The immune-suppressive effects of omega-3 (n-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether n-3 PUFAs regulated T cell receptor (TCR) and peptide-major histocompatibility complex (pMHC) interactions. The expansion of anti-viral CD8+ T cells that endogenously synthesize n-3 PUFAs (FAT-1) dramatically decreased upon lymphocytic choriomeningitis virus (LCMV) infection in vivo. This decrease was not caused by the considerable reduction of TCR expression or the impaired chemotactic activity of T cells. Interestingly, a highly inclined and laminated optical sheet (HILO) microscopic analysis revealed that the TCR motility was notably reduced on the surface of the FAT-1 CD8+ T cells compared to the wild type (WT) CD8+ T cells. Importantly, the adhesion strength of the FAT-1 CD8+ T cells to the peptide-MHC was significantly lower than that of the WT CD8+T cells. Consistent with this result, treatment with docosahexaenoic acid (DHA), one type of n-3 PUFA, significantly decreased CD8+ T cell adhesion to the pMHC. Collectively, our results reveal a novel mechanism through which n-3 PUFAs decrease TCR-pMHC interactions by modulating TCR mobility on CD8+ T cell surfaces.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
- College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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