Development of pH-responsive starch-glycol chitosan nanogels for proapoptotic (KLAKLAK)(2) peptide delivery
- Authors
- Kim, Seong Kyeong; Youn, Yu Seok; Oh, Kyung Taek; Lee, Eun Seong
- Issue Date
- Jul-2017
- Publisher
- SAGE PUBLICATIONS LTD
- Keywords
- Starch-glycol chitosan nanogels; 3-diethylaminopropyl; endosomolytic activity; D-(KLAKLAK)(2) proapoptotic peptide; tumor therapy
- Citation
- JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, v.32, no.4, pp 345 - 354
- Pages
- 10
- Journal Title
- JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
- Volume
- 32
- Number
- 4
- Start Page
- 345
- End Page
- 354
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4283
- DOI
- 10.1177/0883911516676848
- ISSN
- 0883-9115
1530-8030
- Abstract
- In this study, we report pH-responsive polysaccharidic nanogels for cytosolic peptide delivery. We conjugated starch to water-soluble glycol chitosan and pH-responsive 3-diethylaminopropylamine (starch-(glycol chitosan-3-diethylaminopropylamine)). Starch-(glycol chitosan-3-diethylaminopropylamine) self-organizes in aqueous solution, with the glycol chitosan blocks on the hydrophilic outer shell and starch and 3-diethylaminopropylamine blocks in the hydrogel inner core. The experimental results demonstrated that the protonation of 3-diethylaminopropylamine at pH 6.0 (endosomal pH) allowed for accelerated release of the encapsulated D-(KLAKLAK)(2) proapoptotic peptide from the nanogels as a result of electrostatic repulsion between D-(KLAKLAK)(2) and 3-diethylaminopropylamine. A hemolysis test using red blood cell membranes (as an endosomal membrane model) revealed the excellent endosomolytic activity of these nanogels, which likely stems from the proton-sponge effect of 3-diethylaminopropylamine at pH 6.0. As a result, these nanogels resulted in increased KB tumor cell ablation.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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