Prevalence and Characterization of Virulence Genes in Escherichia coli Isolated from Diarrheic Piglets in KoreaPrevalence and Characterization of Virulence Genes in Escherichia coli Isolated from Diarrheic Piglets in Korea
- Authors
- Sung Jae Kim; Woo Kyung Jung; Joonbae Hong; Soo-Jin Yang; Yong Ho Park; Kun Taek Park
- Issue Date
- 2020
- Publisher
- 한국식품위생안전성학회
- Keywords
- Diarrheic piglet; E. coli: Prevalence; Virulence factor
- Citation
- 한국식품위생안전성학회지, v.35, no.3, pp 271 - 278
- Pages
- 8
- Journal Title
- 한국식품위생안전성학회지
- Volume
- 35
- Number
- 3
- Start Page
- 271
- End Page
- 278
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/43017
- DOI
- 10.13103/JFHS.2020.35.3.271
- ISSN
- 1229-1153
2465-9223
- Abstract
- Enterotoxigenic Escherichia coli is one of the major causative infectious agents of diarrhea in newborn and post-weaning pigs and leads to a large economic loss worldwide. However, there is limited information on the distribution and characterization of virulence genes in E. coli isolated from diarrheic piglets, which also applies to the current status of pig farms in Korea. To investigate the prevalence and characterization of virulence genes in E. coli related to diarrhea in piglets, the rectal swab samples of diarrheic piglets (aged 2 d to 6 w) were collected from 163 farms between 2013 and 2016. Five to 10 individual swab samples from the same farm were pooled and cultured on MacConkey agar plates, and E. coli were identified using the API 32E system. Three sets of multiplex PCRs were used to detect 13 E. coli virulence genes. As a result, a total of 172 E. coli isolates encoding one or more of the virulence genes were identified. Among them, the prevalence of individual virulence gene was as follows, (1) fimbrial adhesins (43.0%): F4 (16.9%), F5 (4.1%), F6 (1.7%), F18 (21.5%), and F41 (3.5%); (2) toxins (90.1%): LT (19.2%), STa (20.9%), STb (25.6%), Stx2e (15.1%), EAST1 (48.3%); and (3) non-fimbrial adhesin (19.6%): EAE (14.0%), AIDA-1 (11.6%) and PAA (8.7%), respectively. Taken together, various pathotypes and virotypes of E. coli were identified in diarrheic piglets. These results suggest a broad array of virulence genes is associated with coliform diarrhea in piglets in Korea.
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