Pepper Novel Serine-Threonine Kinase CaDIK1 Regulates Drought ToleranceviaModulating ABA Sensitivity

Abstract

Adaptation to drought stress is essential for plant growth and development. Plants often adapt to water deficit conditions by activating the ABA signaling. Here, we report that the pepperCaDIK1(CapsicumannuumDroughtInducedKinase 1) gene is essential for plant tolerance to drought stress.CaDIK1contains a serine-threonine kinase domain, which plays a role for attachment of phosphate to the target protein. The expression levels ofCaDIK1are upregulated in pepper leaves by ABA, drought, NaCl and H(2)O(2)treatments suggesting its role in abiotic stress response. We usedCaDIK1-silenced pepper andCaDIK1-overexpressing (OX) transgenic Arabidopsis plants to evaluate their responses to ABA and drought.CaDIK1-silenced pepper plants conferred a reduced ABA sensitivity and drought hypersensitivity, which was accompanied by high levels of transpirational water loss.CaDIK1-OX plants displayed opposite phenotypes toCaDIK1-silenced peppers. In contrast, substitution of Lys350 to Asn in the kinase domain of CaDIK1 did not lead to alteration of drought sensitivity. Collectively, these data indicate thatCaDIK1is a positive regulator of the ABA-mediated drought-stress tolerance.