BES1 negatively regulates the expression of ACC oxidase 2 to control the endogenous level of ethylene in Arabidopsis thaliana
- Authors
- Moon, Jinyoung; Kim, Soon Young; Park, Chan-Ho; Kim, Seong-Ki
- Issue Date
- 1-Feb-2021
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- ACC oxidase 2; Arabidopsis thaliana; BES1; brassinosteroids; ethylene production
- Citation
- PLANT SIGNALING & BEHAVIOR, v.16, no.2
- Journal Title
- PLANT SIGNALING & BEHAVIOR
- Volume
- 16
- Number
- 2
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/43759
- DOI
- 10.1080/15592324.2020.1850625
- ISSN
- 1559-2316
1559-2324
- Abstract
- Quantitative reverse transcription PCR (qRT-PCR) analysis and ProACO2::GUS expression showed that ACO2 was highly expressed in the shoots of Arabidopsis seedlings under light conditions. Exogenously applied aminocyclopropane-1-carboxylic acid (ACC) enhanced the expression of ACO2, whereas Co2+ ions suppressed its expression. In comparison with wild-type seedlings, the ACO2 knockdown mutant aco2-1 produced less ethylene, which resulted in the inhibited growth of Arabidopsis seedlings. Exogenously applied brassinolide reduced the expression of ACO2. ACO2 expression was increased in det2, a brassinosteroid (BR)-deficient mutant; however, it was decreased in bes1-D, a brassinosteroid insensitive 1-EMS-suppressor 1 (BES1)-dominant mutant. In the putative promoter region of ACO2, 11 E-box sequences for BES1 binding but not BR regulatory element sequences for brassinazole-resistant 1 (BZR1) binding were found. Chromatin immunoprecipitation assay showed that BES1 could directly bind to the E-boxes located in the putative promoter region of ACO4. Less ethylene was produced in bes1-D seedlings compared with wild-type seedlings, suggesting that the direct binding of BES1 to the ACO2 promoter may negatively regulate ACO2 expression to control the endogenous level of ethylene in Arabidopsis seedlings.
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