Green lipped mussel oil complex suppresses lipopolysaccharide stimulated inflammation via regulating nuclear factor-kappa B and mitogen activated protein kinases signaling in RAW264.7 murine macrophages

Abstract

Here we investigated the effect of green lipped mussel oil complex (GLMOC) on inflammation and underlying mechanism in lipopolysaccharide stimulated RAW264.7 murine macrophage cells. GLMOC containing green lipped mussel oil (GLMO), olive oil, and vitamin E (10:20:1) can induce significant suppression of iNOS, leading to reduced nitric oxide synthesis, and cyclooxygenase-2, leading to reduced prostaglandin E2 synthesis. In addition, it down-regulated the release of pro-inflammatory cytokines, including tumor necrosis factor-alpha, interleukin (IL)-6, and IL-1 beta. Similar to upstream signaling mediators, GLMCO inhibited the degradation of inhibitory kappa B, nuclear translocation of NF-kappa B, and phosphorylation of mitogen activated protein kinases (MAPKs) in a dose-dependent manner. Among the components of GLMOC, GLMO was responsible for anti-inflammatory efficacy. Taken together, GLMOC induces anti-inflammatory activity via regulating NF-kappa B and MAPK signaling in lipopolysaccharide-induced RAW264.7 cells, providing underlying mechanisms that elucidate the anti-inflammatory efficacy of GLMOC.