Integrated hand-powered centrifugation and paper-based diagnosis with blood-in/answer-out capabilities
- Authors
- Li, Bowei; Qi, Ji; Fu, Longwen; Han, Jinglong; Choo, Jaebum; deMello, Andrew J.; Lin, Bingcheng; Chen, Lingxin
- Issue Date
- 1-Oct-2020
- Publisher
- Elsevier Ltd
- Keywords
- Cancer biomarker; Microfluidic paper-based device; On site diagnosis; μPAD
- Citation
- Biosensors and Bioelectronics, v.165
- Journal Title
- Biosensors and Bioelectronics
- Volume
- 165
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/44138
- DOI
- 10.1016/j.bios.2020.112282
- ISSN
- 0956-5663
1873-4235
- Abstract
- To date, 55% of inhabitants of the developing world still live in rural regions and they have a very urgent need to improve the level of disease diagnosis, especially in resource-limited settings. Herein, we present a microfluidic system that centrifuges whole blood and quantifies contained biomarkers in a rapid, feasible and integrated way. Such a device provides a blood-in/answer-out capability and is small enough to be carried by any individual in any environment. The successful integration a hand-powered centrifuge and immunoassay unit within a rotational paper-based device allows for diagnostic application by untrained users and in environments where access to electricity cannot be assumed. In addition, the low cost (~$ 0.5), light weight and small instrumental footprint make the device ideally suited for rapid on-site detection. To validate the applicability of the system in a clinical diagnostic testing, we successfully perform enzyme-linked immunosorbent assay (ELISA) analysis of carcinoembryonic antigen and alpha fetoprotein from human blood samples. We expect that this powerful platform technology will provide the opportunities for point-of-care diagnosis in resource-limited settings. © 2020 Elsevier B.V.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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