Characteristics of Waldenström Macroglobulinemia in Korean Patients According to Mutational Status of MYD88 and CXCR4: Analysis Using Ultra-Deep Sequencing
- Authors
- Shin, D.W.; Kim, S.; Kim, J.; Park, H.S.; Hwang, S.M.; Im, K.; Kim, S.; Kim, J.; Kwon, S.; Yoon, S.-S.; Lee, D.S.
- Issue Date
- Aug-2019
- Publisher
- Elsevier Inc.
- Keywords
- CXCR4; MYD88; Single cell analysis; Ultra-deep sequencing; Waldenström macroglobulinemia
- Citation
- Clinical Lymphoma, Myeloma and Leukemia, v.19, no.8, pp e496 - e505
- Journal Title
- Clinical Lymphoma, Myeloma and Leukemia
- Volume
- 19
- Number
- 8
- Start Page
- e496
- End Page
- e505
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/44738
- DOI
- 10.1016/j.clml.2019.03.009
- ISSN
- 2152-2650
2152-2669
- Abstract
- The frequencies of myeloid differentiation factor 88 (MYD88)/C-X-C chemokine receptor type 4 (CXCR4) mutations and the corresponding clinical characteristics in 31 Korean patients with Waldenström macroglobulinemia were assessed. Ultra-deep sequencing (for CXCR4) and several polymerase chain reaction-based methods (for MYD88) revealed that the frequencies of MYD88/CXCR4 mutations in these Korean patients were similar to reported frequencies for Caucasian individuals. Immunoglobulin M levels and tumor burden were highest in MYD88L265PCXCR4-mutation patients. © 2019 The AuthorsBackground: Little is known about the mutational frequency of myeloid differentiation factor 88 (MYD88) and C-X-C chemokine receptor type 4 (CXCR4) and the corresponding characteristics in Asian individuals afflicted with Waldenström macroglobulinemia (WM). We investigated the characteristics of WM according to mutational status of MYD88/CXCR4, and attempted to determine the lineage commitment among hematopoietic cells by MYD88L265P single-cell sequencing on bone marrow (BM) smear slides. Materials and Methods: CXCR4 mutations (muts) were detected using ultra-deep sequencing using target capture. Mutational burden of MYD88 was assessed using real-time polymerase chain reaction. Single-cell sequencing for MYD88 was performed on lymphocytes, plasmacytoid lymphocytes, plasma cells, and neutrophils using laser microdissection. Results: Among 31 patients, the frequencies of MYD88/CXCR4 muts were as follows: MYD88 wild type (WT) CXCR4WT (6 patients, 19.4%), MYD88L265PCXCR4WT (19 patients, 61.4%), MYD88L265PCXCR4mut (6 patients, 19.4%; 1 frameshift and 5 nonsense muts). Immunoglobulin M levels of MYD88L265CXCR4WT patients were significantly higher than those of MYD88WTCXCR4WT patients (P =.024). Tumor burden in BM was highest in patients with MYD88L265PCXCR4mut (82.0%), followed by MYD88L265PCXCR4WT (52.8%) and MYD88WTCXCR4WT (14.2%) (P <.001). The quantity of MYD88-mutated DNA tended to correlate with tumor burden in BM (correlation coefficient 0.647; P =.009). MYD88L265P was detected in plasma cells, plasmacytoid lymphocytes, and lymphocytes but not neutrophils. Conclusion: The frequency of MYD88/CXCR4 muts in Korean and Caucasian patients with WM was similar, however 5 of the 6 CXCR4 muts were nonsense—a proportion higher than reported frequencies in Caucasian individuals. Ultra-deep sequencing was capable of detecting CXCR4 muts not detectable using Sanger sequencing, suggesting a possible replacement of the B-cell sorting. © 2019 The Authors
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