A novel voxel-wise lesion segmentation technique on 3.0-T diffusion MRI of hyperacute focal cerebral ischemia at 1h after permanent MCAO in rats
- Authors
- Choi, Chi-Hoon; Yi, Kyung Sik; Lee, Sang-Rae; Lee, Youngjeon; Jeon, Chang-Yeop; Hwang, Jinwoo; Lee, Chulhyun; Choi, Sung Sik; Lee, Hong Jun; Cha, Sang-Hoon
- Issue Date
- Aug-2018
- Publisher
- SAGE PUBLICATIONS INC
- Keywords
- Brain ischemia; diffusion-weighted MRI; animal models; brain imaging; acute stroke
- Citation
- JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, v.38, no.8, pp 1371 - 1383
- Pages
- 13
- Journal Title
- JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
- Volume
- 38
- Number
- 8
- Start Page
- 1371
- End Page
- 1383
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45219
- DOI
- 10.1177/0271678X17714179
- ISSN
- 0271-678X
1559-7016
- Abstract
- To assess hyperacute focal cerebral ischemia in rats on 3.0-Tesla diffusion-weighted imaging (DWI), we developed a novel voxel-wise lesion segmentation technique that overcomes intra- and inter-subject variation in apparent diffusion coefficient (ADC) distribution. Our novel technique involves the following: (1) intensity normalization including determination of the optimal type of region of interest (ROI) and its intra- and inter-subject validation, (2) verification of focal cerebral ischemic lesions at 1h with gross and high-magnification light microscopy of hematoxylin-eosin (H&E) pathology, (3) voxel-wise segmentation on ADC with various thresholds, and (4) calculation of dice indices (DIs) to compare focal cerebral ischemic lesions at 1h defined by ADC and matching H&E pathology. The best coefficient of variation was the mode of the left hemisphere after normalization using whole left hemispheric ROI, which showed lower intra- (2.54 +/- 0.72%) and inter-subject (2.67 +/- 0.70%) values than the original. Focal ischemic lesion at 1h after middle cerebral artery occlusion (MCAO) was confirmed on both gross and microscopic H&E pathology. The 83 relative threshold of normalized ADC showed the highest mean DI (DI=0.820 +/- 0.075). We could evaluate hyperacute ischemic lesions at 1h more reliably on 3-Tesla DWI in rat brains.
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