Surface-Modified Mesh Filter for Direct Nucleic Acid Extraction and its Application to Gene Expression Analysis
- Authors
- You, Jae Bem; Kim, Yong Tae; Lee, Kyoung G.; Choi, Yunho; Choi, Seongkyun; Kim, Chi Hyun; Kim, Kyung Hoon; Chang, Sung Jin; Lee, Tae Jae; Lee, Seok Jae; Im, Sung Gap
- Issue Date
- 25-Oct-2017
- Publisher
- WILEY
- Keywords
- cationic polymers; initiated chemical vapor deposition (iCVD); nucleic acid extraction; pathogen detection
- Citation
- ADVANCED HEALTHCARE MATERIALS, v.6, no.20
- Journal Title
- ADVANCED HEALTHCARE MATERIALS
- Volume
- 6
- Number
- 20
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45491
- DOI
- 10.1002/adhm.201700642
- ISSN
- 2192-2640
2192-2659
- Abstract
- Rapid and convenient isolation of nucleic acids (NAs) from cell lysate plays a key role for onsite gene expression analysis. Here, this study achieves one-step and efficient capture of NA directly from cell lysate by developing a cationic surface-modified mesh filter (SMF). By depositing cationic polymer via vapor-phase deposition process, strong charge interaction is introduced on the surface of the SMF to capture the negatively charged NAs. The NA capturing capability of SMF is confirmed by X-ray photoelectron spectroscopy, fluorescent microscopy, and zeta potential measurement. In addition, the genomic DNAs of Escherichia Coli O157:H7 can be extracted by the SMF from artificially infected food, and fluorescent signal is observed on the surface of SMF after amplification of target gene. The proposed SMF is able to provide a more simplified, convenient, and fast extraction method and can be applied to the fields of food safety testing, clinical diagnosis, or environmental pollutant monitoring.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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