Bromopropane Compounds Increase the Stemness of Colorectal Cancer Cells
- Authors
- Cho, Young-Chang; Thanh Thi Nguyen; Park, So-Yeon; Kim, Kwonseop; Kim, Hyung Sik; Jeong, Hye Gwang; Kim, Kyung Keun; Kim, Hangun
- Issue Date
- Sep-2017
- Publisher
- MDPI
- Keywords
- bromopropane; colorectal cancer; cancer stem cell
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.18, no.9
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 18
- Number
- 9
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45524
- DOI
- 10.3390/ijms18091888
- ISSN
- 1422-0067
1422-0067
- Abstract
- Bromopropane (BP) compounds, including 1-bromopropane, 2-bromopropane, and 1,2-dibromopropane, are used in industry for various purposes, and their deleterious effects on human health are becoming known. In this study, we examined the effects of BP compounds on the stemness of colorectal cancer cells. At low, non-cytotoxic concentrations, BP compounds significantly increased spheroid formation in CSC221, DLD1, Caco2, and HT29 cells. In addition, the levels of cancer stem cell markers, such as aldehyde dehydrogenase-1, cluster of differentiation 133 (CD133), CD44, Lgr5, Musashi-1, Ephrin receptor, and Bmi-1 increased after exposure to BP compounds. BP compounds increased the transcriptional activity of the TOPflash and glioma-associated oncogene homolog zinc finger protein (Gli) promoters in reporter assays and increased the expression of Gli-1, Gli-2, Smoothened (SMO), and -catenin by RT-PCR. These results demonstrate for the first time that BP compounds have the potential to promote cancer stemness.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45524)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.