The Effects of Nonporous Silica Nanoparticles on Cultured Human Keratocytesopen access
- Authors
- Yim, Bora; Park, Joo-Hee; Jeong, Hyejoong; Hong, Jinkee; Shin, Young-Joo; Chuck, Roy S.; Park, Choul Yong
- Issue Date
- Jan-2017
- Publisher
- ASSOC RESEARCH VISION OPHTHALMOLOGY INC
- Keywords
- silica; nanoparticle; keratocyte; nanotoxicity; viability; mTOR; autophagy
- Citation
- INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, v.58, no.1, pp 362 - 371
- Pages
- 10
- Journal Title
- INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
- Volume
- 58
- Number
- 1
- Start Page
- 362
- End Page
- 371
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45619
- DOI
- 10.1167/iovs.16-20603
- ISSN
- 0146-0404
1552-5783
- Abstract
- PURPOSE. Silica nanoparticles (SiNPs) are promising carriers for ophthalmic drug delivery. In this study, we investigated the effect of various sizes of nonporous SiNPs on cultured human keratocytes. METHODS. Three different sizes of SiNPs (50, 100, and 150 nm) were manufactured. Primarily cultured human keratocytes were exposed to different concentrations (0, 25, 50, and 100 mu g/mL) of three sizes of SiNPs for up to 72 hours. Intracellular reactive oxygen species (ROS) generation, cellular viability, lactate dehydrogenase (LDH) assay, autophagy, vimentin expression, and mammalian target of rapamycin (mTOR) pathway activation were evaluated. Intracellular distribution of SiNPs was evaluated with transmission electron microscopy. RESULTS. Transmission electron microscopy revealed SiNPs were taken up by keratocytes inside cytoplasmic vacuoles. Neither nuclear entry of SiNPs nor mitochondrial structural damage was observed. Both intracellular ROS generation and LDH level remained unchanged with up to 100 mu g/mL SiNP treatment. Cellular viability was not affected by SiNP treatment. Autophagy showed significant dose-dependent activation with 50- and 100-nm SiNPs. However, mTOR activation remained unchanged. Vimentin expression did not show any significant increase with SiNPs. CONCLUSIONS. Our findings suggested that 50-, 100-, and 150-nm SiNPs did not induce significant cytotoxicity in cultured human keratocytes at concentrations up to 100 mu g/mL.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Engineering > School of Chemical Engineering and Material Science > 1. Journal Articles
- College of Natural Sciences > Department of Chemistry > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45619)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.