Surface-enhanced Raman Scattering-based Competitive Immunoassay of Poly-γ-D-Glutamic Acid for Highly Sensitive Detection of Anthrax Biomarker
- Authors
- Ko, Juhui; Cha, Kiweon; Jeon, Jun Ho; Rhie, Gi-eun; Choi, Jonghoon; Choo, Jaebum
- Issue Date
- Mar-2015
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- Surface-enhanced Raman scattering; Bacillus anthracis; Poly-γ-D-glutamic acid; Competitive immunoassay; Magnetic bead
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.36, no.3, pp 822 - 826
- Pages
- 5
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 36
- Number
- 3
- Start Page
- 822
- End Page
- 826
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45755
- DOI
- 10.1002/bkcs.10154
- ISSN
- 0253-2964
1229-5949
- Abstract
- Anthrax is a highly acute disease caused by the bacterium Bacillus anthracis. In this article, we report a highly sensitive detection technique for pathogenic B. anthracis, based on the use of a surface-enhanced Raman scattering (SERS)-active magnetic sensor. Gold nanoparticles (GNPs) and magnetic beads were used as SERS nanotags and supporting substrates, respectively. Here, an SERS-based competitive immunoassay platform is described for the quantitative evaluation of the anthrax marker. Poly-gamma-D-glutamic acid (PGA) capsules were used as a target biomarker for the highly sensitive detection of B. anthracis becausePGAis known to be closely associated with the pathogenesis of B. anthracis infection. The SERS-based competitive assay reveals an extraordinarily high sensitivity for PGA with a limit of detection of similar to 1.0 ng/mL. This work provides a conceptually new immunoassay prototype for fast and sensitive detection of PGA markers.
- Files in This Item
-
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
- College of ICT Engineering > School of Integrative Engineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.