Colloidal gold nanoparticle conjugates of gefitinib
- Authors
- Lam, Anh Thu Ngoc; Yoon, Jinha; Ganbold, Erdene-Ochir; Singh, Dheeraj K.; Kim, Doseok; Cho, Kwang-Hwi; Lee, So Yeong; Choo, Jaebum; Lee, Kangtaek; Joo, Sang-Woo
- Issue Date
- 1-Nov-2014
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Lung cancer cells; Gold nanoparticle conjugates; Gefitinib; Cell viability; Surface-enhanced Raman scattering
- Citation
- COLLOIDS AND SURFACES B-BIOINTERFACES, v.123, pp 61 - 67
- Pages
- 7
- Journal Title
- COLLOIDS AND SURFACES B-BIOINTERFACES
- Volume
- 123
- Start Page
- 61
- End Page
- 67
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45777
- DOI
- 10.1016/j.colsurfb.2014.08.021
- ISSN
- 0927-7765
1873-4367
- Abstract
- Gefitinib (GF) is a US Food and Drug Administration-approved epidermal growth factor receptor (EGFR)tyrosine kinase inhibitor for treating the lung cancers. We fabricated colloidal gold nanoparticle (AuNP) conjugates of the GF anticancer drug by self-assembly to test their potency against A549, NCI-H460, and NCI-H1975 lung cancer cells. GF adsorption on AuNP surfaces was examined by UV vis absorption spectra and surface-enhanced Raman scattering. Density functional theory calculations were performed to estimate the energetic stabilities of the drug-AuNP composites. The N1 nitrogen atom of the quinazoline ring of GF was calculated to be more stable than the N3 in binding Au cluster atoms. The internalizations of GF-coated AuNPs were examined by transmission electron and dark-field microscopy. A cell viability test of AuNP GF conjugates with the EGFR antibody exhibited much higher reductions than free GF for A549, NCI-H460, and NCI-H1975 lung cancer cells after treatment for 48 h. (C) 2014 Elsevier B.V. All rights reserved.
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