Selective Enrichment of Conjugated Linoleic Acid Isomers in Their Mixtures Using Combined Chemical and Enzymatic Methods
DC Field | Value | Language |
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dc.contributor.author | Kim, Jiwon | - |
dc.contributor.author | Chung, Min-Yu | - |
dc.contributor.author | Choi, Hee-Don | - |
dc.contributor.author | Choi, In-Wook | - |
dc.contributor.author | Kim, In-Hwan | - |
dc.contributor.author | Chun, Hyang Sook | - |
dc.contributor.author | Kim, Byung Hee | - |
dc.date.available | 2019-03-08T08:58:00Z | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 0003-021X | - |
dc.identifier.issn | 1558-9331 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4619 | - |
dc.description.abstract | The aim of this study was to selectively enrich t10,c12-conjugated linoleic acid (t10,c12-CLA) and c9,t11-CLA in commercial CLA mixtures using a combination of urea crystallization and lipase-catalyzed esterification. The objective of the urea fractionation is to remove saturated and monounsaturated fatty acids (FA) from the CLA mixtures. CLA-enriched free FA (FFA) mixtures containing 53.8 wt% t10,c12-CLA and 39.1 wt% c9,t11-CLA were produced from the CLA mixtures containing similar to 34 wt% each of the two CLA isomers by a urea crystallization using methanol and the urea-to-FA weight ratio of 2.5:1. The CLA-enriched FFA mixtures were partially esterified with dodecan-1-ol in a recirculating packed-bed reactor using an immobilized lipase from Candida rugosa to further enrich the t10,c12-CLA and c9,t11-CLA in an FFA fraction and an FA dodecyl ester fraction, respectively, under the optimal conditions, i.e., temperature, 20 A degrees C; FA-to-dodecan-1-ol molar ratio, 1:1; water content, 2 wt% of total substrates; residence time, 5 min; and reaction time, 24 h (for t10,c12-CLA enrichment) and 12 h (for c9,t11-CLA enrichment). After the reaction, an FFA fraction with 72.6 wt% t10,c12-CLA was obtained. Another FFA fraction with 62.0 wt% c9,t11-CLA was recovered after the saponification of the FA dodecyl ester fraction. The yields of t10,c12-CLA and c9,t11-CLA in the FFA fractions were 43.6 and 21.5 wt%, respectively, based on their initial weights in the CLA mixtures. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Selective Enrichment of Conjugated Linoleic Acid Isomers in Their Mixtures Using Combined Chemical and Enzymatic Methods | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s11746-017-2970-6 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, v.94, no.4, pp 577 - 585 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000398849700008 | - |
dc.identifier.scopusid | 2-s2.0-85014201028 | - |
dc.citation.endPage | 585 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 577 | - |
dc.citation.title | JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | - |
dc.citation.volume | 94 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Urea crystallization | - |
dc.subject.keywordAuthor | Esterification | - |
dc.subject.keywordAuthor | Candida rugosa lipase | - |
dc.subject.keywordAuthor | Recirculating packed-bed reactor | - |
dc.subject.keywordAuthor | trans-10,cis-12 Conjugated linoleic acid | - |
dc.subject.keywordAuthor | cis-9,trans-11 Conjugated linoleic acid | - |
dc.subject.keywordPlus | LIPASE-CATALYZED ESTERIFICATION | - |
dc.subject.keywordPlus | CANDIDA-RUGOSA LIPASE | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | BED REACTOR | - |
dc.subject.keywordPlus | CLA ISOMERS | - |
dc.subject.keywordPlus | FRACTIONATION | - |
dc.subject.keywordPlus | TRIACYLGLYCEROLS | - |
dc.subject.keywordPlus | SOLVENT | - |
dc.subject.keywordPlus | OIL | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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