콜레스테롤 산화 생성물 7-Ketocholesterol에 의한 세포자멸사에 미치는 Extracellular Signal-Regulated Kinase 억제의 효과Effect of Extracellular Signal-Regulated Kinase Inhibition on Oxysterol 7-Ketocholesterol-Induced Apoptosis
- Authors
- 황정연; 이선화; 한정호; 김두응; 이정수
- Issue Date
- 2011
- Publisher
- 대한신경과학회
- Keywords
- 7-Ketocholesterol; ERK inhibition; PC12 cells; Apoptosis-related proteins; Cell death; 7-Ketocholesterol; ERK inhibition; PC12 cells; Apoptosis-related proteins; Cell death
- Citation
- 대한신경과학회지, v.29, no.4, pp 317 - 325
- Pages
- 9
- Journal Title
- 대한신경과학회지
- Volume
- 29
- Number
- 4
- Start Page
- 317
- End Page
- 325
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/46731
- ISSN
- 1225-7044
- Abstract
- Background: Defects in mitochondrial function have been shown to participate in the induction of neuronal cell injury.
The extracellular-signal-regulated kinase (ERK) signaling pathway plays a crucial role in almost all cell functions,including proliferation, differentiation, survival, and death. However, the effect of ERK inhibition on oxysterol-induced apoptosis remains uncertain.
Methods: This study assessed the effect of ERK inhibition on the apoptotic effect of 7-ketocholesterol.
Results: Treatment with 7-ketocholesterol increased phosphorylated-ERK1/2 levels in differentiated PC12 cells, while the total amount of ERK was not altered. 7-Ketocholesterol decreased Bid and Bcl-2 levels, increased Bax and p53 levels,and promoted cytochrome c release, which elicits the activation of caspases (-8, -9, and -3), nuclear damage, and cell death. ERK and farnesyltransferase inhibitors inhibited the 7-ketocholesterol-induced phosphorylation of ERK1/2,activation of apoptosis-related proteins, and cell death in PC12 cells.
Conclusions: The ERK and farnesyltransferase inhibitors, which did not exhibit toxicity, may inhibit the 7-ketocholesterol toxicity on differentiated PC12 cells by suppressing the activation of the caspase-8-dependent pathway as well as activation of the mitochondria-mediated cell-death pathway, leading to the activation of caspases. The inhibition of ERK may confer a beneficial protective effect against the neuronal cell injury induced by cholesterol oxidation products.
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