Fabrication of peptide stabilized fluorescent gold nanocluster/graphene oxide nanocomplex and its application in turn-on detection of metalloproteinase-9
DC Field | Value | Language |
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dc.contributor.author | Phuong-Diem Nguyen | - |
dc.contributor.author | Vu Thanh Cong | - |
dc.contributor.author | Baek, Changyoon | - |
dc.contributor.author | Min, Junhong | - |
dc.date.available | 2019-03-08T08:58:41Z | - |
dc.date.issued | 2017-03 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4686 | - |
dc.description.abstract | This study introduces the double-ligands stabilizing gold nanoclusters and the fabrication of gold nanocluster/graphene nanocomplex as a "turn-on" fluorescent probe for the detection of cancer-related enzyme matrix metalloproteinase-9. A facile, one-step approach was developed for the synthesis of fluorescent gold nanoclusters using peptides and mercaptoundecanoic acid as co-templating ligands. The peptide was designed to possess a metalloproteinase-9 cleavage site and to act not only as a stabilizer but also as a targeting ligand for the enzyme detection. The prepared gold nanoclusters show an intense red fluorescence with a broad adsorption spectrum. In the presence of the enzyme, due to the excellent quenching properties and the negligible background of graphene oxide, the developed peptide-gold nanocluster/graphene nanocomplex yielded an intense "turn-on" fluorescent response, which strongly correlated with the enzyme concentration. The limit of detection of the nanocomplex was 0.15 nM. The sensor was successfully applied for "turn-on" detection of metalloproteinase-9 secreted from human breast adenocarcinoma MCF-7 cells with high sensitivity, selectivity, significant improvement in terms of detection time and simplicity. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Fabrication of peptide stabilized fluorescent gold nanocluster/graphene oxide nanocomplex and its application in turn-on detection of metalloproteinase-9 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bios.2015.12.031 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.89, pp 666 - 672 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000391077000072 | - |
dc.identifier.scopusid | 2-s2.0-84951019957 | - |
dc.citation.endPage | 672 | - |
dc.citation.startPage | 666 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 89 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Gold nanocluster | - |
dc.subject.keywordAuthor | Graphene oxide | - |
dc.subject.keywordAuthor | Peptide | - |
dc.subject.keywordAuthor | Metalloproteinase-9 | - |
dc.subject.keywordAuthor | Cancer metastasis | - |
dc.subject.keywordAuthor | Cleavage site | - |
dc.subject.keywordAuthor | Fluorescent quenching | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | PROTEASE ACTIVITY | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | MMP-9 | - |
dc.subject.keywordPlus | GELATINASE | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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