Fabrication of peptide stabilized fluorescent gold nanocluster/graphene oxide nanocomplex and its application in turn-on detection of metalloproteinase-9
- Authors
- Phuong-Diem Nguyen; Vu Thanh Cong; Baek, Changyoon; Min, Junhong
- Issue Date
- Mar-2017
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Gold nanocluster; Graphene oxide; Peptide; Metalloproteinase-9; Cancer metastasis; Cleavage site; Fluorescent quenching
- Citation
- BIOSENSORS & BIOELECTRONICS, v.89, pp 666 - 672
- Pages
- 7
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 89
- Start Page
- 666
- End Page
- 672
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4686
- DOI
- 10.1016/j.bios.2015.12.031
- ISSN
- 0956-5663
1873-4235
- Abstract
- This study introduces the double-ligands stabilizing gold nanoclusters and the fabrication of gold nanocluster/graphene nanocomplex as a "turn-on" fluorescent probe for the detection of cancer-related enzyme matrix metalloproteinase-9. A facile, one-step approach was developed for the synthesis of fluorescent gold nanoclusters using peptides and mercaptoundecanoic acid as co-templating ligands. The peptide was designed to possess a metalloproteinase-9 cleavage site and to act not only as a stabilizer but also as a targeting ligand for the enzyme detection. The prepared gold nanoclusters show an intense red fluorescence with a broad adsorption spectrum. In the presence of the enzyme, due to the excellent quenching properties and the negligible background of graphene oxide, the developed peptide-gold nanocluster/graphene nanocomplex yielded an intense "turn-on" fluorescent response, which strongly correlated with the enzyme concentration. The limit of detection of the nanocomplex was 0.15 nM. The sensor was successfully applied for "turn-on" detection of metalloproteinase-9 secreted from human breast adenocarcinoma MCF-7 cells with high sensitivity, selectivity, significant improvement in terms of detection time and simplicity. (C) 2015 Elsevier B.V. All rights reserved.
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