Microarray detection of duplex and triplex DNA binders with DNA-modified gold nanoparticles
- Authors
- Lytton-Jean, A.K.R.; Min, S.H.; Mirkin, C.A.
- Issue Date
- 1-Aug-2007
- Citation
- Analytical Chemistry, v.79, no.15, pp 6037 - 6041
- Pages
- 5
- Journal Title
- Analytical Chemistry
- Volume
- 79
- Number
- 15
- Start Page
- 6037
- End Page
- 6041
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47005
- DOI
- 10.1021/ac070635h
- ISSN
- 0003-2700
1520-6882
- Abstract
- We have designed a chip-based assay, using microarray technology, for determining the relative binding affinities of duplex and triplex DNA binders. This assay combines the high discrimination capabilities afforded by DNA-modified Au nanoparticles with the high-throughput capabilities of DNA microarrays. The detection and screening of duplex DNA binders are important because these molecules, in many cases, are potential anticancer agents as well as toxins. Triplex DNA binders are also promising drug candidates. These molecules, in conjunction with triplex-forming oligonucleotides, could potentially be used to achieve control of gene expression by interfering with transcription factors that bind to DNA. Therefore, the ability to screen for these molecules in a high-throughput fashion could dramatically improve the drug screening process. The assay reported here provides excellent discrimination between strong, intermediate, and weak duplex and triplex DNA binders in a high-throughput fashion. © 2007 American Chemical Society.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.