자성 미세입자에의 항체 고정화 방법이 면역결합반응에 미치는 영향Effect of Antibody Immobilization Method to Magnetic Micro Beads on its Immunobinding Characteristics
- Authors
- 최효진; 황상연; 장대호; 조형민; 강정혜; 성기훈; 주재범; 이은규
- Issue Date
- Feb-2006
- Publisher
- 한국화학공학회
- Keywords
- Magnetic Particles; Biorecognition of IgG; Oriented Immobilization; Surface Modification
- Citation
- Korean Chemical Engineering Research(HWAHAK KONGHAK), v.44, no.1, pp 65 - 72
- Pages
- 8
- Journal Title
- Korean Chemical Engineering Research(HWAHAK KONGHAK)
- Volume
- 44
- Number
- 1
- Start Page
- 65
- End Page
- 72
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47033
- ISSN
- 0304-128X
2233-9558
- Abstract
- Recent technical advances in the biorecognition engineering and the microparticle fabrication may enable us to develop the single step purification using magnetic particle, because of its simplicity, efficacy, ease of automation, and process economics. In this study, we used commercial magnetic particles from Seradyn, Inc. (Indianapolis, USA). It was ca. 2.8 micron in diameter, consisted of polystyrene core and magnetite coating, and its surface had carboxyl groups. The model, capture protein was IgG and anti-IgG was used as the ligand molecule. We studied the different surfaces ('nude', ester-activated, and anti-IgG coated) for their biorecognition of IgG. At a high pH condition, we could reduce non-specific binding. Also anti-IgG immobilized magnetic particle could capture IgG more selectively. We attempted 'oriented immobilization' of anti-IgG, in which the polysaccharides moiety near the C-terminus was selectively oxidized and linked to the hydrazine-coated MP, to improve the efficacy of biorecognitive binding. Using this method, the IgG capturing ability was improved by ca. 2 fold. From the binary mixture of the IgG-insulin, IgG could be more selectively captured. In summary, the oriented immobilization of oxidized anti-IgG proved to be as effective as the streptavidin-biotin system and yet simpler and cost-effective. This immobilization method can find its applications in protein biochips and biotargeting.
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