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Simultaneous determination of saikosaponin derivatives in Bupleurum falcatum by HPLC-ELSD analysis using different extraction methods

Authors
Choi, J.Kim, J.Kang, S.S.Lee, Sang Hyun
Issue Date
2021
Publisher
한국응용생명화학회
Keywords
Bupleurum falcatum; Extraction method; High performance liquid chromatograph; Quantitative analysis; Saikosaponin
Citation
Journal of Applied Biological Chemistry, v.64, no.1, pp 57 - 61
Pages
5
Journal Title
Journal of Applied Biological Chemistry
Volume
64
Number
1
Start Page
57
End Page
61
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/48750
DOI
10.3839/jabc.2021.009
ISSN
1976-0442
2234-7941
Abstract
Saikosaponin derivatives such as saikosaponins A, B1, B2, B3, B4, C, and D present in Bupleurum falcatum were analyzed by a high performance liquid chromatograph equipped with an evaporative light scattering detector, using different extraction solvents (water and 70% ethanol). The samples were injected into a YMC Pack Pro C18 column and separated using a gradient elution system with a mobile phase composed of acetonitrile and water at a flow rate of 1.1 mL/min. The content of saikosaponin derivatives was higher in 70% ethanol extract than in water extract. This study provides an efficient analytical method for determining the optimal conditions for extraction of saikosaponin derivatives, which can be used as a basis for development of functional foods and pharmaceutical products from B. falcatum.
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