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Simultaneous determination of saikosaponin derivatives in Bupleurum falcatum by HPLC-ELSD analysis using different extraction methods
- Authors
- Choi, J.; Kim, J.; Kang, S.S.; Lee, Sang Hyun
- Issue Date
- 2021
- Publisher
- 한국응용생명화학회
- Keywords
- Bupleurum falcatum; Extraction method; High performance liquid chromatograph; Quantitative analysis; Saikosaponin
- Citation
- Journal of Applied Biological Chemistry, v.64, no.1, pp 57 - 61
- Pages
- 5
- Journal Title
- Journal of Applied Biological Chemistry
- Volume
- 64
- Number
- 1
- Start Page
- 57
- End Page
- 61
- ISSN
- 1976-0442
2234-7941
- Abstract
- Saikosaponin derivatives such as saikosaponins A, B1, B2, B3, B4, C, and D present in Bupleurum falcatum were analyzed by a high performance liquid chromatograph equipped with an evaporative light scattering detector, using different extraction solvents (water and 70% ethanol). The samples were injected into a YMC Pack Pro C18 column and separated using a gradient elution system with a mobile phase composed of acetonitrile and water at a flow rate of 1.1 mL/min. The content of saikosaponin derivatives was higher in 70% ethanol extract than in water extract. This study provides an efficient analytical method for determining the optimal conditions for extraction of saikosaponin derivatives, which can be used as a basis for development of functional foods and pharmaceutical products from B. falcatum.
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