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Simultaneous determination of phytochemical constituents in Paeonia lactiflora extracts using the HPLC-UV methodopen access
- Authors
- Kim, J.; Choi, J.; Kang, S.S.; Lee, Sang Hyun
- Issue Date
- 2021
- Publisher
- 한국응용생명화학회
- Keywords
- Albiflorin · Benzoic acid · (+)-Catechin · Gallic acid methyl ester · Paeonia lactiflora · Paeoniflorin ·Paeonol · Quantitative analysis
- Citation
- Journal of Applied Biological Chemistry, v.64, no.1, pp 13 - 17
- Pages
- 5
- Journal Title
- Journal of Applied Biological Chemistry
- Volume
- 64
- Number
- 1
- Start Page
- 13
- End Page
- 17
- ISSN
- 1976-0442
2234-7941
- Abstract
- Qantitative analysis of six compounds: (+)-catechin, benzoic acid, gallic acid methyl ester, paeonol, paeoniflorin, and albiflorin from Paeonia lactiflora extracts was performed using high-performance liquid chromatography and an ultraviolet (UV) detector, following different extraction methods. A reverse-phase column was used in a gradient elution system, and UV detection was performed at 280 nm. The results showed that the quantity of paeoniflorin was the highest in ethanol and water extracts (73.89 and 57.87 mg/g, respectively) among the six compounds. This study contributes a good analysis method for the contents of P. lactiflora and would be propitious for developing medicines and functional foods.
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