Advances in Accurate Microbial Genome- Editing CRISPR TechnologiesAdvances in Accurate Microbial Genome- Editing CRISPR Technologies
- Authors
- Lee, Ho Joung; Lee, Sang Jun
- Issue Date
- Jul-2021
- Publisher
- 한국미생물·생명공학회
- Keywords
- CRISPR; Cas9; genome editing; bacteria; mismatch intolerance
- Citation
- Journal of Microbiology and Biotechnology, v.31, no.7, pp 903 - 911
- Pages
- 9
- Journal Title
- Journal of Microbiology and Biotechnology
- Volume
- 31
- Number
- 7
- Start Page
- 903
- End Page
- 911
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/48926
- DOI
- 10.4014/jmb.2106.06056
- ISSN
- 1017-7825
1738-8872
- Abstract
- Previous studies have modified microbial genomes by introducing gene cassettes containing selectable markers and homologous DNA fragments. However, this requires several steps including homologous recombination and excision of unnecessary DNA regions, such as selectable markers from the modified genome. Further, genomic manipulation often leaves scars and traces that interfere with downstream iterative genome engineering. A decade ago, the CRISPR/Cas system (also known as the bacterial adaptive immune system) revolutionized genome editing technology.
Among the various CRISPR nucleases of numerous bacteria and archaea, the Cas9 and Cas12a (Cpf1) systems have been largely adopted for genome editing in all living organisms due to their simplicity, as they consist of a single polypeptide nuclease with a target-recognizing RNA. However, accurate and fine-tuned genome editing remains challenging due to mismatch tolerance and protospacer adjacent motif (PAM)-dependent target recognition. Therefore, this review describes how to overcome the aforementioned hurdles, which especially affect genome editing in higher organisms.
Additionally, the biological significance of CRISPR-mediated microbial genome editing is discussed, and future research and development directions are also proposed.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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