Characterization of a novel endolysin from bacteriophage infectingVibrio parahaemolyticus,vB_VpaP_KF2
DC Field | Value | Language |
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dc.contributor.author | Lim, Jeong-A | - |
dc.contributor.author | Lee, Nari | - |
dc.contributor.author | Chun, Hyang-Sook | - |
dc.contributor.author | Chang, Hyun-Joo | - |
dc.date.accessioned | 2021-08-27T07:40:19Z | - |
dc.date.available | 2021-08-27T07:40:19Z | - |
dc.date.issued | 2020-07-21 | - |
dc.identifier.issn | 2468-0834 | - |
dc.identifier.issn | 2468-0842 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/48952 | - |
dc.description.abstract | The antimicrobial resistance of food-borne pathogenic bacteria, includingVibrio parahaemolyticus,has been reported globally, warranting the need to identify promising alternative antibiotics such as endolysins that originate from bacteriophages. In our previous study, we characterized a bacteriophage infectingV. parahaemolyticus, vB_VpaP_KF2, at the molecular level. In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in theEscherichia coliexpression system. The recombinant endolysin, vB_VpaP_KF2_Lys, exhibited a novel lytic property against Gram-negative bacteria regardless of pretreatment with an outer-membrane permeabilizer. It was also stable over a wide range of temperatures, pH, and NaCl concentrations, and its hydrolytic spectrum was broader than that of the parent bacteriophage. From the results, vB_VpaP_KF2_Lys could be used as a biocontrol agent against food-borne pathogens in the field of food safety. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER SINGAPORE PTE LTD | - |
dc.title | Characterization of a novel endolysin from bacteriophage infectingVibrio parahaemolyticus,vB_VpaP_KF2 | - |
dc.title.alternative | Characterization of a novel endolysin from bacteriophage infecting Vibrio parahaemolyticus, vB_VpaP_KF2 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s13765-020-00523-z | - |
dc.identifier.bibliographicCitation | APPLIED BIOLOGICAL CHEMISTRY, v.63, no.1, pp 1 - 5 | - |
dc.identifier.kciid | ART002620400 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000550780700001 | - |
dc.identifier.scopusid | 2-s2.0-85088385605 | - |
dc.citation.endPage | 5 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | APPLIED BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 63 | - |
dc.type.docType | Article | - |
dc.publisher.location | 싱가폴 | - |
dc.subject.keywordAuthor | Bacteriophage | - |
dc.subject.keywordAuthor | Endolysin | - |
dc.subject.keywordAuthor | Lytic activity | - |
dc.subject.keywordAuthor | Vibrio parahaemolyticus | - |
dc.subject.keywordAuthor | Food safety | - |
dc.subject.keywordPlus | VIBRIO-PARAHAEMOLYTICUS | - |
dc.subject.keywordPlus | ANTIBIOTIC-RESISTANCE | - |
dc.subject.keywordPlus | GENOME | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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