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Characterization of a novel endolysin from bacteriophage infectingVibrio parahaemolyticus,vB_VpaP_KF2

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dc.contributor.authorLim, Jeong-A-
dc.contributor.authorLee, Nari-
dc.contributor.authorChun, Hyang-Sook-
dc.contributor.authorChang, Hyun-Joo-
dc.date.accessioned2021-08-27T07:40:19Z-
dc.date.available2021-08-27T07:40:19Z-
dc.date.issued2020-07-21-
dc.identifier.issn2468-0834-
dc.identifier.issn2468-0842-
dc.identifier.urihttps://scholarworks.bwise.kr/cau/handle/2019.sw.cau/48952-
dc.description.abstractThe antimicrobial resistance of food-borne pathogenic bacteria, includingVibrio parahaemolyticus,has been reported globally, warranting the need to identify promising alternative antibiotics such as endolysins that originate from bacteriophages. In our previous study, we characterized a bacteriophage infectingV. parahaemolyticus, vB_VpaP_KF2, at the molecular level. In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in theEscherichia coliexpression system. The recombinant endolysin, vB_VpaP_KF2_Lys, exhibited a novel lytic property against Gram-negative bacteria regardless of pretreatment with an outer-membrane permeabilizer. It was also stable over a wide range of temperatures, pH, and NaCl concentrations, and its hydrolytic spectrum was broader than that of the parent bacteriophage. From the results, vB_VpaP_KF2_Lys could be used as a biocontrol agent against food-borne pathogens in the field of food safety.-
dc.format.extent5-
dc.language영어-
dc.language.isoENG-
dc.publisherSPRINGER SINGAPORE PTE LTD-
dc.titleCharacterization of a novel endolysin from bacteriophage infectingVibrio parahaemolyticus,vB_VpaP_KF2-
dc.title.alternativeCharacterization of a novel endolysin from bacteriophage infecting Vibrio parahaemolyticus, vB_VpaP_KF2-
dc.typeArticle-
dc.identifier.doi10.1186/s13765-020-00523-z-
dc.identifier.bibliographicCitationAPPLIED BIOLOGICAL CHEMISTRY, v.63, no.1, pp 1 - 5-
dc.identifier.kciidART002620400-
dc.description.isOpenAccessN-
dc.identifier.wosid000550780700001-
dc.identifier.scopusid2-s2.0-85088385605-
dc.citation.endPage5-
dc.citation.number1-
dc.citation.startPage1-
dc.citation.titleAPPLIED BIOLOGICAL CHEMISTRY-
dc.citation.volume63-
dc.type.docTypeArticle-
dc.publisher.location싱가폴-
dc.subject.keywordAuthorBacteriophage-
dc.subject.keywordAuthorEndolysin-
dc.subject.keywordAuthorLytic activity-
dc.subject.keywordAuthorVibrio parahaemolyticus-
dc.subject.keywordAuthorFood safety-
dc.subject.keywordPlusVIBRIO-PARAHAEMOLYTICUS-
dc.subject.keywordPlusANTIBIOTIC-RESISTANCE-
dc.subject.keywordPlusGENOME-
dc.relation.journalResearchAreaFood Science & Technology-
dc.relation.journalWebOfScienceCategoryFood Science & Technology-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
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