CaADIP1-dependent CaADIK1-kinase activation is required for abscisic acid signalling and drought stress response in Capsicum annuum
- Authors
- Jeong, Soongon; Lim, Chae Woo; Lee, Sung Chul
- Issue Date
- Sep-2021
- Publisher
- WILEY
- Keywords
- abscisic acid; dephosphorylation; drought; kinase; pepper; phosphatase; phosphorylation
- Citation
- NEW PHYTOLOGIST, v.231, no.6, pp 2247 - 2261
- Pages
- 15
- Journal Title
- NEW PHYTOLOGIST
- Volume
- 231
- Number
- 6
- Start Page
- 2247
- End Page
- 2261
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/49008
- DOI
- 10.1111/nph.17544
- ISSN
- 0028-646X
1469-8137
- Abstract
- Induction of the abscisic acid (ABA) signalling network is associated with various stress conditions, including cold, high salinity and drought. As core ABA signalling components, group A type 2C protein phosphatases (PP2Cs) interact with and inhibit snf1-related protein kinase2s. Here, we isolated and characterised the pepper mitogen-activated protein kinase kinase kinase CaADIK1, which interacts with the group A PP2C CaADIP1. CaADIK1 transcripts were induced by abiotic stresses, and CaADIK1 localised in the nucleus and cytoplasm. We verified that CaADIP1 inhibits the autokinase activity of CaADIK1; moreover, the kinase activity of CaADIK1 is enhanced by drought stress. We performed genetic analysis using CaADIK1-silenced pepper and CaADIK1-overexpressing (OX) Arabidopsis plants. CaADIK1-silenced pepper plants showed drought-sensitive phenotypes, whereas CaADIK1-OX Arabidopsis plants showed ABA-sensitive and drought-tolerant phenotypes. In CaADIK1(K32N)-OX Arabidopsis plants mutated at the ATP-binding site, the ABA-insensitive and drought-sensitive phenotypes were restored. Taken together, our findings show that CaADIK1 positively regulates the ABA-dependent drought stress response and is inhibited by CaADIP1.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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