Endocrine disrupting potential of veterinary drugs by in vitro stably transfected human androgen receptor transcriptional activation assays
- Authors
- Park, Y.; Park, J.; Lee, H.-S.
- Issue Date
- Oct-2021
- Publisher
- Elsevier Ltd
- Keywords
- Agonist; Antagonist; Human androgen receptor; OECD test guideline; S9 fraction
- Citation
- Environmental Pollution, v.286
- Journal Title
- Environmental Pollution
- Volume
- 286
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/49517
- DOI
- 10.1016/j.envpol.2021.117201
- ISSN
- 0269-7491
1873-6424
- Abstract
- We describe the androgen receptor (AR) agonistic/antagonistic effects of 140 veterinary drugs regulated in Republic of Korea, by setting maximum residue limits. It was conducted using two in vitro test guidelines of the Organization for Economic Cooperation and Development (OECD)—the AR-EcoScreen AR transactivation (TA) assay and the 22Rv1/MMTV_GR-KO AR TA assay. These were performed alongside the AR binding affinity assay to confirm whether their AR agonistic/antagonistic effects are based on the binding affinity to AR. Prior to conducting the AR TA assay, the proficiency test was passed the proficiency performance criterion for the AR agonist and AR antagonist assays. Among the veterinary drugs tested, four veterinary drugs (dexamethasone, trenbolone, altrenogest, and nandrolone) and six veterinary drugs (cymiazole, dexamethasone, zeranol, phenothiazine, bromopropylate, and isoeugenol) were determined as AR agonist and AR antagonist, respectively in both in vitro AR TA assays. Zeranol exhibited weak AR agonistic effects with a PC10 value only in the 22Rv1/MMTV_GR-KO AR TA assay. Regarding changing the AR agonistic/antagonistic effects through metabolism, the AR antagonistic activities of zeranol, phenothiazine, and isoeugenol decreased significantly in the presence of phase I + II enzymes. These data indicate that various veterinary drugs could have the potential to disrupt AR-mediated human endocrine system. Furthermore, this is the first report providing information on AR agonistic/antagonistic effects of veterinary drugs using in vitro OECD AR TA assays. © 2021 Elsevier Ltd
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School of Education > ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/49517)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.