Snuella sedimenti sp. nov., isolated from marine sediment

Abstract

A Gram stain-negative, aerobic, motile by gliding, rod-shaped bacterial strain CAU 1569(T) was isolated from marine sediment on Shido Island in Incheon. It grew at 20-37 degrees C (optimum, 30 degrees C), pH 6.0-9.0 (optimum, 7.0), 2-6% NaCl (w/v) (optimum, 2%). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CAU 1569(T) formed a distinct lineage with only the type strain of Snuella. Strain CAU 1569(T) showed high similarity to S. lapsa KACC 14152(T) (95.8%), Mariniflexile gromovii KMM KCTC 12570(T), Aestuariibaculum marinum KCTC 52521(T) (95.4%), A. suncheonense KACC 16186(T) (94.6%) and Yeosuana aromativorans KCCM 42019(T) (94.4%). The genome contained 57 contigs, 3,437 protein-coding gene, 3 rRNAs (5, 16, and 23S), 43 tRNAs, and with a 35.7 mol% G + C content. The DDH value between strain CAU 1569(T) and S. lapsa KACC 14152(T) was 39.4 +/- 0.6%. The only isoprenoid quinone was menaquinone 6 (MK-6). The major fatty acids were iso-C-15:0,C- C(15:1-)iso G, and C-17:0 iso 3-OH. Strain CAU 1569(T) contained diphosphatidylglycerol, aminoglycolipid, unidentified aminolipid, and three unidentified lipids. Based on phylogenetic, genomic, physiologic, and chemotaxonomic characterizations, strain CAU 1569(T) represents a novel Snuella species, which the name Snuella sedimenti sp. nov. is proposed. The type of strain is CAU 1569(T) (= KCTC 82409(T) = MCCC 1K05670(T)).