Rapid, multiplexed, and nucleic acid amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor
DC Field | Value | Language |
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dc.contributor.author | Kashefi-Kheyrabadi, Leila | - |
dc.contributor.author | Nguyen, Huynh Vu | - |
dc.contributor.author | Go, Anna | - |
dc.contributor.author | Baek, Changyoon | - |
dc.contributor.author | Jang, Nayoon | - |
dc.contributor.author | Lee, Jiwon Michelle | - |
dc.contributor.author | Cho, Nam-Hyuk | - |
dc.contributor.author | Min, Junhong | - |
dc.contributor.author | Lee, Min-Ho | - |
dc.date.accessioned | 2021-10-05T04:40:10Z | - |
dc.date.available | 2021-10-05T04:40:10Z | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50069 | - |
dc.description.abstract | Considering the worldwide health crisis associated with highly contagious severe respiratory disease of COVID-19 outbreak, the development of multiplexed, simple and rapid diagnostic platforms to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is in high demand. Here, a nucleic acid amplification-free electrochemical biosensor based on four-way junction (4-WJ) hybridization is presented for the detection of SARS-CoV-2. To form a 4-WJ structure, a Universal DNA-Hairpin (UDH) probe is hybridized with two adaptor strands and a SARS-CoV-2 RNA target. One of the adaptor strands is functionalized with a redox mediator that can be detected using an electrochemical biosensor. The biosensor could simultaneously detect 5.0 and 6.8 ag/μL of S and Orf1ab genes, respectively, within 1 h. The biosensor was evaluated with 21 clinical samples (16 positive and 5 negative). The results revealed a satisfactory agreement with qRT-PCR. In conclusion, this biosensor has the potential to be used as an on-site, real-time diagnostic test for COVID-19. © 2021 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier Ltd | - |
dc.title | Rapid, multiplexed, and nucleic acid amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bios.2021.113649 | - |
dc.identifier.bibliographicCitation | Biosensors and Bioelectronics, v.195 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000705179600002 | - |
dc.identifier.scopusid | 2-s2.0-85115150704 | - |
dc.citation.title | Biosensors and Bioelectronics | - |
dc.citation.volume | 195 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | COVID-19 | - |
dc.subject.keywordAuthor | Electrochemical biosensor | - |
dc.subject.keywordAuthor | Orf1ab gene | - |
dc.subject.keywordAuthor | S gene | - |
dc.subject.keywordAuthor | SARS-CoV-2 RNA | - |
dc.subject.keywordPlus | Biosensors | - |
dc.subject.keywordPlus | Cardiology | - |
dc.subject.keywordPlus | Diagnosis | - |
dc.subject.keywordPlus | Diseases | - |
dc.subject.keywordPlus | Polymerase chain reaction | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | COVID-19 | - |
dc.subject.keywordPlus | Electrochemical biosensor | - |
dc.subject.keywordPlus | Health crisis | - |
dc.subject.keywordPlus | High demand | - |
dc.subject.keywordPlus | Nucleic acids amplification | - |
dc.subject.keywordPlus | Orf1ab gene | - |
dc.subject.keywordPlus | S gene | - |
dc.subject.keywordPlus | Severe acute respiratory syndrome coronavirus | - |
dc.subject.keywordPlus | Severe acute respiratory syndrome coronavirus 2 RNA | - |
dc.subject.keywordPlus | Simple++ | - |
dc.subject.keywordPlus | Genes | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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