Rapid, multiplexed, and nucleic acid amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor
- Authors
- Kashefi-Kheyrabadi, Leila; Nguyen, Huynh Vu; Go, Anna; Baek, Changyoon; Jang, Nayoon; Lee, Jiwon Michelle; Cho, Nam-Hyuk; Min, Junhong; Lee, Min-Ho
- Issue Date
- Jan-2022
- Publisher
- Elsevier Ltd
- Keywords
- COVID-19; Electrochemical biosensor; Orf1ab gene; S gene; SARS-CoV-2 RNA
- Citation
- Biosensors and Bioelectronics, v.195
- Journal Title
- Biosensors and Bioelectronics
- Volume
- 195
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50069
- DOI
- 10.1016/j.bios.2021.113649
- ISSN
- 0956-5663
1873-4235
- Abstract
- Considering the worldwide health crisis associated with highly contagious severe respiratory disease of COVID-19 outbreak, the development of multiplexed, simple and rapid diagnostic platforms to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is in high demand. Here, a nucleic acid amplification-free electrochemical biosensor based on four-way junction (4-WJ) hybridization is presented for the detection of SARS-CoV-2. To form a 4-WJ structure, a Universal DNA-Hairpin (UDH) probe is hybridized with two adaptor strands and a SARS-CoV-2 RNA target. One of the adaptor strands is functionalized with a redox mediator that can be detected using an electrochemical biosensor. The biosensor could simultaneously detect 5.0 and 6.8 ag/μL of S and Orf1ab genes, respectively, within 1 h. The biosensor was evaluated with 21 clinical samples (16 positive and 5 negative). The results revealed a satisfactory agreement with qRT-PCR. In conclusion, this biosensor has the potential to be used as an on-site, real-time diagnostic test for COVID-19. © 2021 Elsevier B.V.
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