Tumor Targeting Chitosan Nanoparticles for Dual-Modality Optical/MR Cancer Imaging
- Authors
- Nam, Taehwan; Park, Sangjin; Lee, Seung-Young; Park, Kyeongsoon; Choi, Kuiwon; Han, Moon Hee; Song, In Chan; Leary, James J.; Yuk, Simseok Andrew; Kwon, Ick Chan; Kim, Kwangmeyung; Jeong, Seo Young
- Issue Date
- Apr-2010
- Publisher
- AMER CHEMICAL SOC
- Citation
- BIOCONJUGATE CHEMISTRY, v.21, no.4, pp 578 - 582
- Pages
- 5
- Journal Title
- BIOCONJUGATE CHEMISTRY
- Volume
- 21
- Number
- 4
- Start Page
- 578
- End Page
- 582
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50134
- DOI
- 10.1021/bc900408z
- ISSN
- 1043-1802
1520-4812
- Abstract
- We report tumor targeting nanoparticles for optical/MR dual imaging based on self-assembled glycol chitosan to be a potential multimodal imaging probe. To develop an optical/MR dual imaging probe, biocompatible and water-soluble glycol chitosan (M-w = 50 kDa) were chemically modified with 5 beta-cholanic acid (CA), resulting in amphiphilic glycol chitosan-5 beta-cholanic acid conjugates (GC-CA). For optical imaging near-infrared fluorescence (NIRF) dye, Cy5.5, was conjugated to GC-CA resulting in Cy5-labeled GC-CA conjugates (Cy5.5-GC-CA). Moreover, in order to dictate gadolinium (Gd(III)) in the Cy5.5-GC-CA conjugates, 1,4,7,10-tetraazacyclododecane1,4,7,10-tetraacetic acid (DOTA) was directly conjugated in Cy5.5-GC-CA. Finally, the excess GdCl3 was added to DOTA modified Cy5.5-GC-CA conjugates in distilled water (pH 5.5). The freshly prepared Gd(III) encapsulated Cy5.5-GC-CA conjugates were spontaneously self-assembled into stable Cy5.5 labeled and Gd(III) encapsulated chitosan nanoparticles (Cy5.5-CNP-Gd(III)). The Cy5.5-CNP-Gd(III) was spherical in shape and approximately 350 nm in size. From the cellular experiment, it was demonstrated that Cy5.5-CNP-Gd(III) were efficiently taken up and distributed in cytoplasm (NIRF lilter; red). When the Cy5.5-GC-Gd(III) were systemically administrated into the tail vein of tumor-bearing mice, large amounts of nanoparticles were successfully localized within the tumor, which was confirmed by noninvasive near-infrared fluorescence and MR imaging system simultaneously. These results revealed that the dual-modal imaging probe of Cy5.5-CNP-Gd(III) has the potential to be used as an optical/MR dual imaging agent for cancer treatment.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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