The attenuation of experimental lung metastasis by a bile acid acylated-heparin derivative
- Authors
- Park, Kyeongsoon; Lee, Seok Ki; Son, Dai Hyun; Park, Soo Ah; Kim, Kwangmeyung; Chang, Hyo Won; Jeong, Eun-jeong; Park, Rang-Woon; Kim, In-San; Kwon, Ick Chan; Byun, Youngro; Kim, Sang Yoon
- Issue Date
- Jun-2007
- Publisher
- ELSEVIER SCI LTD
- Keywords
- bile acid acylated-heparin derivative; B16F10 melanoma; adhesion; invasion; lung metastasis
- Citation
- BIOMATERIALS, v.28, no.16, pp 2667 - 2676
- Pages
- 10
- Journal Title
- BIOMATERIALS
- Volume
- 28
- Number
- 16
- Start Page
- 2667
- End Page
- 2676
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50497
- DOI
- 10.1016/j.biomaterials.2007.02.001
- ISSN
- 0142-9612
1878-5905
- Abstract
- The inhibitory efficacies of new bile acid acylated-heparin derivative (heparin-DOCA) were evaluated on experimental lung metastasis. We evaluated the effect of heparin-DOCA on intercellular interactions including those between B16F10 and thrombin-activated platelets and TNF-alpha-activated HUVECs, and between B16F10 and immobilized mouse P-selectin. In addition, the inhibitory effects of heparin-DOCA on adhesion and invasion of B16F10 to Matrigel were studied. In an animal mouse study, the blood clot formation and the retention of red fluorescence protein (RFP)-B16F10 in lungs were assessed after heparin-DOCA and RFP-B16F10 intravenous administration. Furthermore, we investigated the anti-metastatic effect of heparin-DOCA against lung metastasis induced by B16F10 and SCC7. Heparin-DOCA inhibited intercellular interactions between B16F10 and activated platelets or activated HUVECs by blocking P- and E-selectin-mediated interactions. Moreover, it reduced adhesion and invasion of B16F10 to ECM, thereby affecting the reduction of early retention of B16F10 in the lung. Heparin-DOCA attenuated lung colony formation on the surfaces and in interior of the lung, and attenuated metastasis by B16F10 and SCC7. These results suggest that heparin-DOCA may have potentials as therapeutic agent that prevents tumor metastasis and progression. (c) 2007 Elsevier Ltd. All rights reserved.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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