Efficacy of horse oil on lipopolysaccharide-induced inflammation in human keratinocytes

Abstract

OBJECTIVE: To investigate the efficacy of horse oil on lipopolysaccharide (LPS)-induced inflammation in human keratinocytes. METHODS: Western blot analysis was performed to measure the expression of cyclooxygenase-2 (COX-2) and I kappa Ba. ELISA was used to analyze prostaglandin E2 (PGE2) levels. RESULTS: Horse oil decreased LPS-induced COX-2 and PGE2 levels in a dose-dependent manner. Nuclear factor-kappa B (NF-kappa B) plays a key role in the expression of inflammatory cytokines and mediators. Therefore, we investigated the influence of horse oil on the NF-kappa B signaling pathways. Horse oil inhibited translocation of NF-kappa B from the cytosol to the nucleus. Furthermore, LPS-induced degradation of I kappa Ba was recovered by horse oil. The activation of p38 mitogen-activated protein kinase (MAPK) reportedly induces degradation of I kappa Ba In agreement with this, LPS activated p38 MAPK and caused I kappa Ba degradation. Conversely, horse oil inhibited LPS-induced p38 MAPK activation and I kappa Ba degradation. In addition, a specific p38 MAPK inhibitor, SB203580, blocked I kappa Ba degradation. CONCLUSION: Horse oil decreased COX-2 and PGE2 by inhibiting p38 MAPK activation, I kappa Ba degradation, and the translocation of NF-kappa B. (C) 2021 JTCM. All rights reserved.