Methods of measuring presynaptic function with fluorescence probesMethods of measuring presynaptic function with fluorescence probes
- Authors
- 장예슬; 김성래; 이성훈
- Issue Date
- 2021
- Publisher
- 한국현미경학회
- Keywords
- Synaptic vesicles Exo- and endocytosis Presynaptic terminal Fluorescence probes
- Citation
- 한국현미경학회지, v.51, no.1, pp 1 - 7
- Pages
- 7
- Journal Title
- 한국현미경학회지
- Volume
- 51
- Number
- 1
- Start Page
- 1
- End Page
- 7
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50649
- DOI
- 10.1186/s42649-021-00051-0
- ISSN
- 2287-5123
2287-4445
- Abstract
- Synaptic vesicles, which are endogenous to neurotransmitters, are involved in exocytosis by active potentials and release neurotransmitters. Synaptic vesicles used in neurotransmitter release are reused via endocytosis to maintain a pool of synaptic vesicles. Synaptic vesicles show different types of exo- and endocytosis depending on animal species, type of nerve cell, and electrical activity. To accurately understand the dynamics of synaptic vesicles, direct observation of synaptic vesicles is required; however, it was difficult to observe synaptic vesicles of size 40–50 nm in living neurons. The exo-and endocytosis of synaptic vesicles was confirmed by labeling the vesicles with a fluorescent agent and measuring the changes in fluorescence intensity. To date, various methods of labeling synaptic vesicles have been proposed, and each method has its own characteristics, strength, and drawbacks. In this study, we introduce methods that can measure presynaptic activity and describe the characteristics of each technique.
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