HPLC/DAD Method Validation of 6-Hydroxyluteolin 7-O-Glucoside Analysis from Salvia plebeia곰보배추에 함유된 6-Hydroxyluteolin 7-O-Glucoside 분석을 위한 HPLC/DAD 분석법 밸리데이션
- Authors
- 이학동; 바헤; 최중원; 김주리; 유아람; 배민정; 이상현
- Issue Date
- 2021
- Publisher
- 한국생약학회
- Keywords
- Salvia plebeia; 6-Hydroxyluteolin 7-O-glucoside; Method validation; HPLC/DAD
- Citation
- 생약학회지, v.52, no.3, pp 186 - 191
- Pages
- 6
- Journal Title
- 생약학회지
- Volume
- 52
- Number
- 3
- Start Page
- 186
- End Page
- 191
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51239
- DOI
- 10.22889/KJP.2021.52.3.186
- ISSN
- 0253-3073
- Abstract
- We developed a method to identify and quantify 6-hydroxyluteolin 7-O-glucoside in the powder of Salvia plebeia (PS) using high-performance liquid chromatography coupled with diode array detector (HPLC/DAD) and equipped with reverse-phase INNO C18 column. The analytical method was optimized and validated using novel parameters. The obtained values for the limits of detection and quantification were 3.60 and 10.90 μg/mL, respectively. Calibration curve showed good linearity in the concentration range tested (0.00625-0.1 mg/mL, r2 = 1.0000), high accuracy (96.2-101.4%), and precision values (RSD £ 0.27%). Our analysis support the use of our method for accurately identifying and quantifying 6-hydroxyluteolin 7-O-glucoside from PS in routine analyses and large-scale extraction processes for content determination.
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