Bone Formation in a Rat Tibial Defect Model Using Carboxymethyl Cellulose/BioC/Bone Morphogenic Protein-2 Hybrid Materials
- Authors
- Song, Sang-Heon; Yun, Young-Pil; Kim, Hak-Jun; Park, Kyeongsoon; Kim, Sung Eun; Song, Hae-Ryong
- Issue Date
- 2014
- Publisher
- HINDAWI LTD
- Citation
- BIOMED RESEARCH INTERNATIONAL, v.2014
- Journal Title
- BIOMED RESEARCH INTERNATIONAL
- Volume
- 2014
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51442
- DOI
- 10.1155/2014/230152
- ISSN
- 2314-6133
2314-6141
- Abstract
- The objective of this study was to assess whether carboxymethyl cellulose-(CMC-) based hydrogel containing BioC (biphasic calcium phosphate (BCP); tricalcium phosphate (TCP) : hydroxyapatite (Hap) = 70 : 30) and bone morphogenic protein-2 (BMP-2) led to greater bone formation than CMC-based hydrogel containing BioC without BMP-2. In order to demonstrate bone formation at 4 and 8 weeks, plain radiographs, microcomputed tomography (micro-CT) evaluation, and histological studies were performed after implantation of all hybrid materials on an 8mm defect of the right tibia in rats. The plain radiographs and micro-CT analyses revealed that CMC/BioC/BMP-2 (0.5mg) led to much greater mineralization at 4 and 8 weeks than did CMC/BioC or CMC/Bio/BMP-2 (0.1mg). Likewise, bone formation and bone remodeling studies revealed that CMC/BioC/BMP-2 (0.5mg) led to a significantly greater amount of bone formation and bone remodeling at 4 and 8 weeks than did CMC/BioC or CMC/BioC/BMP-2 (0.1mg). Histological studies revealed that mineralized bone tissue was present around the whole circumference of the defect site with CMC/BioC/BMP-2 (0.5mg) but not with CMC/BioC or CMC/BioC/BMP-2 (0.1mg) at 4 and 8weeks. These results suggest that CMC/BioC/BMP-2 hybrid materials induced greater bone formation than CMC/BioC hybrid materials. Thus, CMC/BioC/BMP-2 hybrid materials may be used as an injectable substrate to regenerate bone defects.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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