Clinical trial: Magnetoplasmonic elisa for urine-based active tuberculosis detection and anti-tuberculosis therapy monitoring
DC Field | Value | Language |
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dc.contributor.author | Kim, Jeonghyo | - |
dc.contributor.author | Tran, Van Tan | - |
dc.contributor.author | Oh, Sangjin | - |
dc.contributor.author | Jang, Minji | - |
dc.contributor.author | Lee, Dong Kun | - |
dc.contributor.author | Hong, Jong Chul | - |
dc.contributor.author | Park, Tae Jung | - |
dc.contributor.author | Kim, Hwa-Jung | - |
dc.contributor.author | Lee, Jaebeom | - |
dc.date.accessioned | 2021-11-25T07:40:11Z | - |
dc.date.available | 2021-11-25T07:40:11Z | - |
dc.date.issued | 2021-11 | - |
dc.identifier.issn | 2374-7943 | - |
dc.identifier.issn | 2374-7951 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51848 | - |
dc.description.abstract | The coronavirus disease 2019 (COVID-19) pandemic has proved the importance of fast and widespread diagnostic testing to prevent serious epidemics timely. The first-line weapon against rapidly transmitted disease is a quick and massive screening test to isolate patients immediately, preventing dissemination. Here, we described magnetoplasmonic nanozymes (MagPlas NZs), i.e., hierarchically coassembled Fe3O4-Au superparticles, that are capable of integrating magnetic enrichment and catalytic amplification, thereby the assay can be streamlined amenable to high-throughput operation and achieve ultrahigh sensitivity. Combining this advantage with conventional enzyme-linked immunosorbent assay (ELISA), we propose a MagPlas ELISA for urine-based tuberculosis (TB) diagnosis and anti-TB therapy monitoring, which enables fast (<3 h), and highly sensitive (up to pM with naked-eyes, < 10 fM with plate reader) urinary TB antigen detection. A clinical study with a total of 297 urine samples showed robust sensitivity for pulmonary tuberculosis (85.0%) and extra-pulmonary tuberculosis (52.8%) patients with high specificity (96.7% and 96.9%). Furthermore, this methodology offers a great promise of noninvasive therapeutic response monitoring, which is impracticable in the gold-standard culture method. The MagPlas ELISA showed high sensitivity comparable to the PCR assay while retaining a simple and cheap ELISA concept, thus it could be a promising point-of-care test for TB epidemic control and possibly applied to other acute infections. © 2021 The Authors. Published by American Chemical Society | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Clinical trial: Magnetoplasmonic elisa for urine-based active tuberculosis detection and anti-tuberculosis therapy monitoring | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acscentsci.1c00948 | - |
dc.identifier.bibliographicCitation | ACS Central Science, v.7, no.11, pp 1898 - 1907 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000722705100016 | - |
dc.identifier.scopusid | 2-s2.0-85119018679 | - |
dc.citation.endPage | 1907 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1898 | - |
dc.citation.title | ACS Central Science | - |
dc.citation.volume | 7 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | MAGNETOPHORETIC IMMUNOASSAY | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | EFFICIENT | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | FACILE | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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