Clinical trial: Magnetoplasmonic elisa for urine-based active tuberculosis detection and anti-tuberculosis therapy monitoring
- Authors
- Kim, Jeonghyo; Tran, Van Tan; Oh, Sangjin; Jang, Minji; Lee, Dong Kun; Hong, Jong Chul; Park, Tae Jung; Kim, Hwa-Jung; Lee, Jaebeom
- Issue Date
- Nov-2021
- Publisher
- American Chemical Society
- Citation
- ACS Central Science, v.7, no.11, pp 1898 - 1907
- Pages
- 10
- Journal Title
- ACS Central Science
- Volume
- 7
- Number
- 11
- Start Page
- 1898
- End Page
- 1907
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51848
- DOI
- 10.1021/acscentsci.1c00948
- ISSN
- 2374-7943
2374-7951
- Abstract
- The coronavirus disease 2019 (COVID-19) pandemic has proved the importance of fast and widespread diagnostic testing to prevent serious epidemics timely. The first-line weapon against rapidly transmitted disease is a quick and massive screening test to isolate patients immediately, preventing dissemination. Here, we described magnetoplasmonic nanozymes (MagPlas NZs), i.e., hierarchically coassembled Fe3O4-Au superparticles, that are capable of integrating magnetic enrichment and catalytic amplification, thereby the assay can be streamlined amenable to high-throughput operation and achieve ultrahigh sensitivity. Combining this advantage with conventional enzyme-linked immunosorbent assay (ELISA), we propose a MagPlas ELISA for urine-based tuberculosis (TB) diagnosis and anti-TB therapy monitoring, which enables fast (<3 h), and highly sensitive (up to pM with naked-eyes, < 10 fM with plate reader) urinary TB antigen detection. A clinical study with a total of 297 urine samples showed robust sensitivity for pulmonary tuberculosis (85.0%) and extra-pulmonary tuberculosis (52.8%) patients with high specificity (96.7% and 96.9%). Furthermore, this methodology offers a great promise of noninvasive therapeutic response monitoring, which is impracticable in the gold-standard culture method. The MagPlas ELISA showed high sensitivity comparable to the PCR assay while retaining a simple and cheap ELISA concept, thus it could be a promising point-of-care test for TB epidemic control and possibly applied to other acute infections. © 2021 The Authors. Published by American Chemical Society
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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